Metabolism Of RNA | Research Area | St John's Laboratory

Western blot analysis of various cells using ERAB Polyclonal Antibody

Anti-HSD17B10 antibody (111-160 aa) (STJ92970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using ERAB/HSD17B10 Rabbit monoclonal antibody (STJ11101265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HSD17B10 antibody (162-261) [S5MR] (STJ11101265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-HSP-27 antibody [G3.1] (STJA0018378)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human

£1,347.50

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Anti-HSP27/HSPB1 antibody [SRM15038] (STJ11109768)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/IHC-P/ELISA

Reactivity :

Human

£0.00

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Anti-HSP70 antibody (STJ13100154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset/Rat/Mouse

£331.50

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Western blot analysis of HSP70 at dilution of 1:1000

Anti-HSP70 antibody [SM0470] (STJA0010470)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-HSP70 antibody [SM4959] (STJA0004959)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-HSP70 antibody [SM5081] (STJA0005081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunofluorescence analysis of U2OS cells using HSP70 Rabbit polyclonal antibody (STJ114814) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPA1A antibody (500-600) (STJ114814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot of T47D cell lysate treated with EGF (1 nM) for 60 minutes showing specific labeling of the ~70 kDa Hsp70 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is eliminated or significantly decreased with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-HSPA1A antibody (STJA0003671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£454.50

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Western blot analysis of extracts of various cell lines, using HSP70/HSPA1 antibody (STJ24092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HSPA1A antibody [S2MM] (STJ24092)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various cells using HSC 70 Polyclonal Antibody diluted at 1：2000

Anti-HSPA8 antibody (202-251 aa) (STJ93596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of L929 cells using Hsc70/HSPA8 Rabbit polyclonal antibody (STJ24099) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPA8 antibody (510-615) (STJ24099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Hsc70/HSPA8 Rabbit monoclonal antibody (STJ11101237) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HSPA8 antibody (547-646) [S7MR] (STJ11101237)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of 1) Hela, 2) HepG2, 3) Raw, 4) Mouse Brain, 5) Rat Brain, 6) Rat Liver using HSC 70 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-HSPA8 antibody (588-638) (STJA0006703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human BrainTissue using HSC70 mouse Monoclonal antibody diluted at 1:200.

Anti-HSPA8 antibody [7G12] (STJ196659)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Western blot analysis of lysates from Hep G2 cells using HSP27/HSPB1 Rabbit pAb (STJ24101) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HSPB1 antibody (1-100) (STJ24101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells using HSP27/HSP27/HSPB1 antibody (STJ118760) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPB1 antibody (1-120) (STJ118760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Confocal imaging of NIH/3T3 cells using HSP27/HSPB1 Rabbit monoclonal antibody (STJ11101283, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HSPB1 antibody (100-205) [S3MR] (STJ11101283)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of mouse-liver tissue. 1, HSP27 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-HSPB1 antibody (48-97 aa) (STJ93617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using HSP27 monoclonal antibody against HeLa (1) , A549 (2) , Jurkat (3) , A431 (4) , HEK293 (5) , HepG2 (6) and PC-12 (7) cell lysate.

Anti-HSPB1 antibody [5D7] (STJ98141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat

£149.00 - £360.00

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Western blot detection of Hsp27 in Hela, HsHela, A431, COS7, Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted).Predicted band size: 23KDa.Observed band size:27KDa.

Anti-HSPB1 antibody [7E5-1F9-D9] (STJ99170)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Monkey

£149.00 - £360.00

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Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2, 000

Anti-HSPB1 antibody [Mix-mA] (STJ97485)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using HTATSF1 antibody (STJ27773) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HTATSF1 antibody (1-270) (STJ27773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HTATSF1 antibody (STJA0007375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using IMP1 antibody (STJ11104772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IGF2BP1 antibody (1-100) (STJ11104772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-IGF2BP1 antibody (10-90 aa) (STJ190314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using IGF2BP1/IMP1 antibody (STJ24140) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IGF2BP1 antibody (478-577) (STJ24140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IGF2BP1 antibody [PT1248R] (STJA0040155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-IGF2BP1/IMP1 antibody [SRM14839] (STJ11109569)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of HuvEC cells using IGF2-BP2 Polyclonal Antibody

Anti-IGF2BP2 antibody (141-190 aa) (STJ93649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of paraffin-embedded rat testis using IGF2BP2/IMP2 Rabbit polyclonal antibody (STJ116159) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IGF2BP2 antibody (330-599) (STJ116159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using IGF2BP2/IGF2BP2/IMP2 rabbit monoclonal antibody (STJ11102288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IGF2BP2 antibody (500-595) [S8MR] (STJ11102288)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using IGF2BP2/IMP2 antibody (STJ116038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-IGF2BP2 antibody (500-599) (STJ116038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using IGF2BP2/IMP2 antibody (STJ24141) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IGF2BP2 antibody (500-599) (STJ24141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (STJ24142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IGF2BP3 antibody (1-200) (STJ24142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using IGF2BP3 antibody (STJ116296) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IGF2BP3 antibody (300-579) (STJ116296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (STJ24143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IGF2BP3 antibody (300-579) (STJ24143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot detection of IMP3 in MCF7, SKVO3, NTERA2, Jurkat, Hela and K562 cell lysates using IMP3 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-IGF2BP3 antibody [1F1-E10-D11] (STJ99190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using IMP-3 monoclonal antibody against Jurkat (1) , K562 (2) and NTERA-2 (3) cell lysate.

Anti-IGF2BP3 antibody [8F11] (STJ98176)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-IGF2BP3/IMP3 antibody [SRM14624] (STJ11109354)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates using IK Rabbit polyclonal antibody (STJ117475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15S.

Anti-IK antibody (1-280) (STJ117475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using IK Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-IK antibody (508-557 aa) (STJ93662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HEPG2 cells using Antibody diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-IMP3 antibody (135-184 aa) (STJ98644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IMP3 antibody [IHC606] (STJA0042193)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-p

Reactivity :

Human

£152.50 - £423.50

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Anti-IMP4 antibody (STJA0008354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ISG20L2 antibody (STJ191860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using ISY1 Rabbit polyclonal antibody (STJ115770) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ISY1 antibody (1-307) (STJ115770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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