Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PCSK1 antibody (125-175 aa) (STJ194864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of rat brain, using PCSK1 antibody (STJ114128) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PCSK1 antibody (28-170) (STJ114128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-PCSK2 antibody (STJA0007829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug PCSK9 Rabbit polyclonal antibody antibody (STJ11105346 1:100). Western blot was performed from the immunoprecipitate using PCSK9 Rabbit polyclonal antibody antibody (STJ11105346) at a dilition of 1:1000.

Anti-PCSK9 antibody (31-692) (STJ11105346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Proprotein Convertase 9 (PCSK9) Rabbit monoclonal antibody (STJ11104600) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PCSK9 antibody (500-600) [S4600RM] (STJ11104600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using Proprotein Convertase 9 (PCSK9) Rabbit pAb (STJ110170) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-PCSK9 antibody (590-692) (STJ110170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of HepG2 cells, using Proprotein Convertase 9 (PCSK9) antibody (STJ11101352) at1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PCSK9 antibody (593-692) [S2MR] (STJ11101352)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PCSK9 antibody (STJA0010204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human KidneyTissue using PSCK9 mouse Monoclonal antibody diluted at 1:200.

Anti-PCSK9 antibody [5G4] (STJ196699)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human

£180.00 - £261.00

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Anti-PCSK9 antibody [SAA0158] (STJA0026981)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£254.50 - £329.50

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Anti-PCSK9 antibody [SAA0158] {APC} (STJA0029626)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£310.50 - £405.50

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Anti-PCSK9 antibody [SAA0158] {FITC} (STJA0028660)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£254.50 - £329.50

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Anti-PCSK9 antibody [SAA0158] {PerCP} (STJA0030592)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£292.50 - £379.50

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Anti-PCSK9 antibody [SAA0158] {PE} (STJA0031558)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£310.50 - £405.50

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Anti-PCSK9 antibody [SAA0159] (STJA0026980)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£254.50 - £329.50

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Anti-PCSK9 antibody [SAA0159] {APC} (STJA0029627)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£310.50 - £405.50

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Anti-PCSK9 antibody [SAA0159] {FITC} (STJA0028661)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£254.50 - £329.50

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Anti-PCSK9 antibody [SAA0159] {PerCP} (STJA0030593)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£292.50 - £379.50

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Anti-PCSK9 antibody [SAA0159] {PE} (STJA0031559)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£310.50 - £405.50

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Anti-PCSK9 antibody [SRM14811] (STJ11109541)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-PCSK9 antibody {ABflo® 647} (STJ11108935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Anti-PCSK9 nanobody [SAA0995] (STJN000287)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£209.50 - £1,333.50

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Anti-PCSK9 nanobody [SAA1344] (STJN000286)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£209.50 - £1,333.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDCL antibody (18-68 aa) (STJ194143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PDHA1 antibody (STJ11104699) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PDHA1 antibody (248-348) [S4699RM] (STJ11104699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of PDHA1 in paraffin-embedded human thyroid cancer using PDHA1 Rabbit polyclonal antibody (STJ24939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PDHA1 antibody (30-390) (STJ24939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000

Anti-PDHA1 antibody (314-363 aa) (STJ95006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Confocal imaging of C2C12 cells using PDHA1 Rabbit monoclonal antibody (STJ11101429, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PDHA1 antibody (50-150) [S9MR] (STJ11101429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using PDH-E1 Alpha monoclonal antibody against A549, HeLa cell lysate.

Anti-PDHA1 antibody (C-Term) (STJ98528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

£149.00 - £360.00

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Anti-PDHA1 antibody (STJA0010259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.

Anti-PDHA1 antibody [3H2-F8-B5] (STJ99056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of extracts of various cell lines, using PDHB antibody (STJ29023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDHB antibody (1-300) (STJ29023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHB antibody (160-240 aa) (STJ191235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of PDHB in paraffin-embedded rat kidney tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-PDHB antibody (260-359) [S3MR] (STJ11102123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human placenta using ERp57 Rabbit monoclonal antibody (STJ11102061) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA3 antibody (100-200) [S1MR] (STJ11102061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of A549, HT29, K562 cells using ERp57 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PDIA3 antibody (111-160 aa) (STJ96585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using ERp57 antibody (STJ24942) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA3 antibody (206-505) (STJ24942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Endogenous ERP57-endoplasmic reticulum lumen marker detected at 1:500 dilution; lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PDIA3 antibody (300aa C-Term) (STJ140035)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Anti-PDIA3 antibody (300aa C-Term) (STJ140036)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Confocal imaging of HeLa cells using PDIA6 Rabbit monoclonal antibody (STJ11102028, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-PDIA6 antibody (1-100) [S8MR] (STJ11102028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from wild type (WT) and PDIA6 knockout (KO) 293T cells, using [KO Validated] PDIA6 Rabbit polyclonal antibody (STJ11100065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PDIA6 antibody (20-300) (STJ11100065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using PDIA6 Antibody (STJ29135) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA6 antibody (20-300) (STJ29135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PDIA6 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PDIA6 antibody (220aa C-Term) (STJ140160)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-PDIA6 antibody (STJA0006902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using PDK1/PDHK1 Rabbit monoclonal antibody (STJ11105443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PDK1 antibody (1-100) [S5443RM] (STJ11105443)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDK1 antibody (337-436) (STJ24945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using PDK1/PDHK1 antibody (STJ11104825) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDK1 antibody (337-436) [S4825RM] (STJ11104825)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates, using PDK1/PDHK1 Rabbit mAb (STJ11102396) at 1:20000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PDK1 antibody (337-436) [S6MR] (STJ11102396)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options