• Western blot analysis of extracts of various cell lines, using MPI antibody (STJ29458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MPI antibody (1-423) (STJ29458)

SKU:
STJ29458

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MPI (1-423) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MPI
Gene ID: 4351
Uniprot ID: MPI_HUMAN
Immunogen Region: 1-423
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human MPI (NP_002426.1).
Immunogen Sequence: MAAPRVFPLSCAVQQYAWGK MGSNSEVARLLASSDPLAQI AEDKPYAELWMGTHPRGDAK ILDNRISQKTLSQWIAENQD SLGSKVKDTFNGNLPFLFKV LSVETPLSIQAHPNKELAEK LHLQAPQHYPDANHKPEMAI ALTPFQGLCGFRPVEEIVTF LKKVPEFQFLIGDEAATHLK QTMSHDSQAVASSLQSCFSH LMKSEKKVVVEQLNLLVKRI SQQAAAGNNMEDIFGELLL
Tissue Specificity Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
Function Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Protein Name Mannose-6-Phosphate Isomerase
Phosphohexomutase
Phosphomannose Isomerase
Pmi
Database Links Reactome: R-HSA-4043916
Reactome: R-HSA-446205
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Mannose-6-Phosphate Isomerase antibody
Anti-Phosphohexomutase antibody
Anti-Phosphomannose Isomerase antibody
Anti-Pmi antibody
Anti-MPI antibody
Anti-PMI1 antibody

Information sourced from Uniprot.org

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