Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CAPZB antibody (STJ110405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CAPZB antibody (1-272) (STJ110405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CAPZB antibody (STJA0007368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using CARD 11 Polyclonal Antibody

Anti-CARD11 antibody (10-59 aa) (STJ92000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CARD11 rabbit monoclonal antibody (STJ11102516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CARD11 antibody (1055-1154) [S6MR] (STJ11102516)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of A549 cells using CARD11 antibody (STJ29220). Blue: DAPI for nuclear staining.

Anti-CARD11 antibody (985-1154) (STJ29220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CARD9 antibody (193-492 aa) (STJ11107411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of CARD9 in K562, THP-1 lysates using CARD9 antibody.

Anti-CARD9 antibody (467-480 aa) [R02-7H5] (STJA0011067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£246.50

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Anti-CARD9 antibody (STJA0004760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Anti-CARD9 antibody (STJA0004761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Anti-CARD9 antibody (STJA0006941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of Casein Kinase 2 beta (CSNK2B) in paraffin-embedded mouse testis using Casein Kinase 2 beta (Casein Kinase 2 beta (CSNK2B) ) Rabbit polyclonal antibody (STJ11102908) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Casein Kinase 2 beta antibody (1-215) (STJ11102908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of THP-1 cells, using Caspase-1 antibody (STJ119181) at 1:500 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CASP1 antibody (120-297) (STJ119181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Human tonsil tissue using CASP1 Rabbit polyclonal antibody (STJ11102943) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.

Anti-CASP1 antibody (198-297) (STJ11102943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using Caspase-1 Rabbit polyclonal antibody (STJ11104266) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CASP1 antibody (198-297) (STJ11104266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of THP-1, using Caspase-1 p20 Rabbit monoclonal antibody (STJ11105107) at 1:2000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP1 antibody (200-300) [S5107RM] (STJ11105107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunofluorescence analysis of U2OS cells using Caspase-1 Rabbit polyclonal antibody (STJ22893) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CASP1 antibody (285-404) (STJ22893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) 293T, 2) Hela, 3) MCF-7, 4) Hela-UV cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CASP1 antibody (350-400 aa) (STJ98574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Caspase-1 (PTR1290) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1Predicted band size: 45, 42, 35, 12, 10kDaObserved band size: 45kDa

Anti-CASP1 antibody (350-404) [PTR1290] (STJA0006259)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Horse/Pig

£48.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Caspase-1 (PTR1317) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1Predicted band size: 45, 42, 35, 12, 10kDaObserved band size: 45kDa

Anti-CASP1 antibody (350-404) [PTR1317] (STJA0006272)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Horse/Pig

£48.00 - £252.00

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Western blot analysis of extracts of Jurkat, using Caspase-10 Rabbit polyclonal antibody (STJ22895) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CASP10 antibody (220-521) (STJ22895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using Caspase-10 Polyclonal Antibody (STJ22894) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CASP10 antibody (230-505) (STJ22894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from Jurkat, K562 cells, using Caspase-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CASP10 antibody (Internal) (STJ96459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Caspase-2 Polyclonal Antibody

Anti-CASP2 antibody (123-172 aa) (STJ92020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CASP2 Polyclonal Antibody (STJ28291) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CASP2 antibody (153-452) (STJ28291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Caspase-2 polyclonal antibody (STJ11102181) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP2 antibody (334-452) [S1MR] (STJ11102181)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of Jurkat cells, using Active Caspase-3 Rabbit monoclonal antibody (STJ112894) at 1:500 dilution. Jurkat cells were treated by Staurosporine (1 Mu M) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP3 antibody (1-100) [S4MR] (STJ112894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using pro Caspase 3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CASP3 antibody (1-20 aa) [R06-1B2] (STJA0010811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£246.50

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Western blot analysis of various cells using Caspase-3 Polyclonal Antibody diluted at 1：1000

Anti-CASP3 antibody (116-165 aa) (STJ92021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Fish

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using Caspase-3 p12 antibody (STJ11101753). HeLa cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (176-277) [S3MR] (STJ11101753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from wild type (WT) and active + pro Caspase-3 knockout (KO) 293T cells, using [KO Validated] active + pro Caspase-3 Rabbit monoclonal antibody (STJ11101749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP3 antibody (29-175) [S9MR] (STJ11101749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of various lysates using Caspase-3 Mouse monoclonal antibody (STJ119900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP3 antibody (55-160) [S0MM] (STJ119900)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of U2OS cells using [KO Validated] Caspase-3 Rabbit polyclonal antibody (STJ113869) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (55-277) (STJ113869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of PC-12 cells using Caspase 3 antibody (STJ22899) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (55-277) (STJ22899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] Caspase-3 rabbit polyclonal antibody (STJ22900) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CASP3 antibody (55-277) (STJ22900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-CASP3 antibody (6-105) (STJ113073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of Caspase 3 (green) in 293 using Caspase 3 antibody, and DAPI (blue).

Anti-CASP3 antibody (75-94 aa) [R02-7C2] (STJA0010817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£264.50

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Anti-CASP3 antibody (N-Term) [M377] (STJA0005295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£319.50

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Anti-CASP3 antibody [31A1067] (STJA0005296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£319.50

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Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP3 antibody [5E1] (STJ97448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IP/IHC

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Immunohistochemistry analysis of Caspase-4 antibody in paraffin-embedded human lung carcinoma tissue.

Anti-CASP4 antibody (75-124 aa) (STJ92022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using Caspase-4 Rabbit polyclonal antibody (STJ11100597) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASP4 antibody (81-270) (STJ11100597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CASP8 antibody (200-300) [S2MR] (STJ11101692)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ113797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP8 antibody (300-400) (STJ113797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ29787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Casp8 antibody (376-479) (STJ29787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CASP8 antibody (C-Term) [M382] (STJA0005298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£319.50

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57 Yang B, Wang L, Luo X, et al. SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis[J]. International journal of oncology, 2015, 46 (2) : 649-656.

Anti-CASP8 antibody (STJ193171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat/Pig/Chicken

£48.00 - £252.00

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Western blot analysis using Caspase-8 monoclonal antibody against HeLa (1) , Jurkat (2) , THP-1 (3) , NIH/3T3 (4) , Cos7 (5) and PC-12 (6) cell lysate.

Anti-CASP8 antibody [1H11] (STJ97895)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP8 antibody [2G12] (STJ97394)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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