Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using IRAK2 antibody (STJ28718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IRAK2 antibody (1-270) (STJ28718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded rat kidney using IRAK2 Rabbit monoclonal antibody (STJ11102125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IRAK2 antibody (50-250) [S5MR] (STJ11102125)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various cells using IRAK-2 Polyclonal Antibody diluted at 1：2000

Anti-IRAK2 antibody (Internal) (STJ93757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-IRAK2 antibody [R06-1X1] (STJA0036874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates using IRAK3 Rabbit polyclonal antibody (STJ115364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IRAK3 antibody (367-596) (STJ115364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HL-60 cells using IRAK3 Rabbit polyclonal antibody (STJ27420) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IRAK3 antibody (367-596) (STJ27420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IRAK3 antibody (450-500) (STJ501491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-IRAK3 antibody (450-500) {Biotin} (STJ501492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-IRAK3 antibody (450-500) {FITC} (STJ501493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of LOVO cells using IRAK-M Polyclonal Antibody

Anti-IRAK3 antibody (491-540 aa) (STJ93758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of THP-1 cells using IRAK4 Rabbit polyclonal antibody (STJ11104538) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IRAK4 antibody (1-200) (STJ11104538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts from wild type (WT) and IRAK4 knockdown (KD) 293T (KD) cells, using IRAK4 antibody (STJ11104081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IRAK4 antibody (1-200) [S4081RM] (STJ11104081)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of NIH/3T3 cells using IRAK4 Rabbit polyclonal antibody (STJ27964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IRAK4 antibody (1-460) (STJ27964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IRAK4 antibody (STJA0009927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IRAK4 antibody (STJA0010261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IRAK4 antibody [R01-1B2] (STJA0034484)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-IRAK4 antibody [SAA1623] (STJA0020051)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-IRAKM antibody [R06-6C2] (STJA0036030)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts of Rat spleen, using IRF1 antibody (STJ110005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IRF1 antibody (181-280) (STJ110005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of PC12 cells using IRF-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-IRF1 antibody (91-140 aa) (STJ97352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IRF1 antibody [R08-1B4] (STJA0034486)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using IRF2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IRF2 antibody (101-150 aa) (STJ93760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g IRF2 antibody (STJ11102165). Western blot was performed from the immunoprecipitate using IRF2 (STJ11102165) at a dilution of 1:500.

Anti-IRF2 antibody (250-349) [S5MR] (STJ11102165)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-IRF2 antibody [R03-7F1] (STJA0036133)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (STJ112965) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IRF3 antibody (100-200) (STJ112965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g IRF3 antibody (STJ24226). Western blot was performed from the immunoprecipitate using IRF3 antibody (STJ24226) at a dilution of 1:1000.

Anti-IRF3 antibody (100-392) (STJ24226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IRF3 antibody (108-166aa) [3F10] (STJA0041509)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-IRF3 antibody (108-166aa) [3F10] (STJA0041933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£238.50 - £361.50

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Immunofluorescence analysis of HeLa cells, using IRF3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IRF3 antibody (351-400 aa) (STJ93761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Pig

£49.00 - £261.00

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Western blot analysis of various cells using IRF-3 Polyclonal Antibody diluted at 1：500

Anti-IRF3 antibody (362-411 aa) (STJ93763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IRF3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-IRF3 antibody (414-427 aa) [R02-6B0] (STJA0012434)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates using [KO Validated] IRF3 Rabbit monoclonal antibody (STJ11101781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IRF3 antibody (50-150) [S1MR] (STJ11101781)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

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Anti-IRF3 antibody [5D2] (STJA0032658)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Monkey

£224.50 - £308.50

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Immunohistochemical analysis of paraffin-embedded Human Spleen Tissue using IRF3 mouse Monoclonal antibody diluted at 1:200.

Anti-IRF3 antibody [5H10] (STJ196651)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-IRF3 antibody [PT0376R] (STJA0039580)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-IRF3 antibody [SRM14968] (STJ11109698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates using IRF4 Rabbit polyclonal antibody (STJ24228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IRF4 antibody (150-350) (STJ24228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using IRF-4 Polyclonal Antibody

Anti-IRF4 antibody (281-330 aa) (STJ93764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MUM1 (ABT080) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: DaudiPredicted band size: 52kDaObserved band size: 52kDa

Anti-IRF4 antibody (350-451 aa) [ABT080] (STJ197173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

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Anti-IRF4 antibody (STJA0037759)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Western blot analysis of extracts of various cell lines, using IRF5 antibody (STJ24230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-IRF5 antibody (1-200) (STJ24230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-IRF5 antibody (176-240aa) [10T1] (STJA0041508)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/IHC/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-IRF5 antibody (176-240aa) [10T1] (STJA0041891)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£238.50 - £361.50

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Anti-IRF5 antibody (380-460 aa) (STJ190152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse thymus using 3 Mu g IRF5 antibody (STJ11101278). Western blot was performed from the immunoprecipitate using IRF5 antibody (STJ11101278) at a dilution of 1:500.

Anti-IRF5 antibody (6-151) [S8MR] (STJ11101278)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-IRF5 antibody [R03-5E9] (STJA0036123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using IRF6 antibody (STJ28002) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 15s.

Anti-IRF6 antibody (115-300) (STJ28002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g IRF6 antibody (STJ11101837). Western blot was performed from the immunoprecipitate using IRF6 antibody (STJ11101837) at a dilution of 1:1000.

Anti-IRF6 antibody (368-467) [S7MR] (STJ11101837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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