• Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g IRF6 antibody (STJ11101837). Western blot was performed from the immunoprecipitate using IRF6 antibody (STJ11101837) at a dilution of 1:1000.
  • Immunofluorescence analysis of NIH-3T3 cells using IRF6 Rabbit monoclonal antibody (STJ11101837) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using IRF6 Rabbit monoclonal antibody (STJ11101837) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using IRF6 Rabbit monoclonal antibody (STJ11101837) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IRF6 Rabbit monoclonal antibody (STJ11101837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IRF6 antibody (368-467) [S7MR] (STJ11101837)

SKU:
STJ11101837

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IRF6 (368-467) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRF6
Gene ID: 3664
Uniprot ID: IRF6_HUMAN
Immunogen Region: 368-467
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 368-467 of human IRF6 (O14896).
Immunogen Sequence: PFEIYLCFGEEWPDGKPLER KLILVQVIPVVARMIYEMFS GDFTRSFDSGSVRLQISTPD IKDNIVAQLKQLYRILQTQE SWQPMQPTPSMQLPPALPPQ
Tissue Specificity Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
Post Translational Modifications Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
Function Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development. Plays a role in regulating mammary epithelial cell proliferation. May regulate WDR65 transcription.
Protein Name Interferon Regulatory Factor 6
Irf-6
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-909733
Cellular Localisation Nucleus
Cytoplasm
Translocates To Nucleus In Response To An Activating Signal
Alternative Antibody Names Anti-Interferon Regulatory Factor 6 antibody
Anti-Irf-6 antibody
Anti-IRF6 antibody

Information sourced from Uniprot.org

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