Anti-IRF5 antibody (1-200) (STJ24230)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IRF5 (1-200) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IRF5 |
| Gene ID | 3663 |
| Uniprot ID | IRF5_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MNQSIPVAPTPPRRVRLKPW LVAQVNSCQYPGLQWVNGEK KLFCIPWRHATRHGPSQDGD NTIFKAWAKETGKYTEGVDE ADPAKWKANLRCALNKSRDF RLIYDGPRDMPPQPYKIYEV CSNGPAPTDSQPPEDYSFGA GEEEEEEEELQRMLPSLSLT EDVKWPPTLQPPTLRPPTLQ PPTLQPPVVLGPPAPDPSPL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2). |
Additional Info
| Post Translational Modifications | Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription. 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation. |
| Function | Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway. Key transcription factor regulating the IFN response during SARS-CoV-2 infection. |
| Protein Name | Interferon Regulatory Factor 5Irf-5 |
| Database Links | Reactome: R-HSA-877300Reactome: R-HSA-909733Reactome: R-HSA-9860276 |
| Cellular Localisation | CytoplasmNucleusShuttles Between The Nucleus And The Cytoplasm: Upon Activation By The Tlr Adapter Myd88 And Subsequent PhosphorylationTranslocates To The Nucleus |
| Alternative Antibody Names | Anti-Interferon Regulatory Factor 5 antibodyAnti-Irf-5 antibodyAnti-IRF5 antibody |
Information sourced from Uniprot.org