Immune System | Research Area | St John's Laboratory

Western blot analysis of Mouse spleen, using CD123 Rabbit polyclonal antibody (STJ11104716) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL3RA antibody (20-305) (STJ11104716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Western blot analysis of HepG2 cells using IL-3R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL3RA antibody (241-290 aa) (STJ97265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse eye, using IL4 Rabbit polyclonal antibody (STJ11102765) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL4 antibody (1-100) (STJ11102765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit polyclonal antibody (STJ27616) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-IL4 antibody (25-153) (STJ27616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IL4 antibody (C-Term) (STJ93700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-IL4 antibody [8D4-8] (STJA0019051)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC/IP/Neut

Reactivity :

Human

£198.50 - £1,170.50

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Anti-IL4 antibody [MP4-25D2] (STJA0002152)

Clonality :

Monoclonal

Host :

Rat

Applications :

Neut/ICFC/IHC

Reactivity :

Human

£185.50 - £2,845.50

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Anti-IL4 antibody [MP4-25D2] (STJA0002153)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£180.50 - £2,642.50

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Anti-IL4 antibody [MP4-25D2] {APC} (STJA0002156)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£131.50 - £303.50

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Anti-IL4 antibody [MP4-25D2] {FITC} (STJA0002154)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£131.50 - £272.50

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Anti-IL4 antibody [MP4-25D2] {PE/TR} (STJA0004015)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC

Reactivity :

Human

£158.50 - £367.50

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Anti-IL4 antibody [MP4-25D2] {PE} (STJA0002155)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£131.50 - £272.50

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Anti-IL4 antibody [SAA0371] (STJA0027550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£98.50

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Anti-IL4 antibody [SAA0371] {APC} (STJA0029011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£201.50

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Anti-IL4 antibody [SAA0371] {FITC} (STJA0028045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£187.50

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Anti-IL4 antibody [SAA0371] {PerCP} (STJA0029977)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£194.50

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Anti-IL4 antibody [SAA0371] {PE} (STJA0030943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£194.50

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Anti-IL4 antibody [SRM13287] (STJ11108017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-IL4 antibody [SRM15101] (STJ11109831)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of Rat lung, using IL4R rabbit polyclonal antibody (STJ116854) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL4R antibody (26-232) (STJ116854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using IL-4R Alpha Polyclonal Antibody diluted at 1：2000

Anti-IL4R antibody (463-512 aa) (STJ93702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using IL5 Rabbit pAb (STJ110003) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 15s.

Anti-IL5 antibody (20-134) (STJ110003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of recombinant Human IL-5 Protein, using Human IL-5 Rabbit polyclonal antibody (STJ11104218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-IL5 antibody (20-134) (STJ11104218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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Western blot analysis of various cells using IL-5 Polyclonal Antibody

Anti-IL5 antibody (43-92 aa) (STJ93704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IL5 antibody [SAA0372] (STJA0027551)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£98.50

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Anti-IL5 antibody [SAA0372] {APC} (STJA0029012)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£201.50

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Anti-IL5 antibody [SAA0372] {FITC} (STJA0028046)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£187.50

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Anti-IL5 antibody [SAA0372] {PerCP} (STJA0029978)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£194.50

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Anti-IL5 antibody [SAA0372] {PE} (STJA0030944)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£194.50

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Anti-IL5 antibody [SAA0373] (STJA0027552)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL5 antibody [SAA0373] {APC} (STJA0029013)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL5 antibody [SAA0373] {FITC} (STJA0028047)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL5 antibody [SAA0373] {PerCP} (STJA0029979)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL5 antibody [SAA0373] {PE} (STJA0030945)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL5 antibody [SAA0374] (STJA0027553)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL5 antibody [SAA0374] {APC} (STJA0029014)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL5 antibody [SAA0374] {FITC} (STJA0028048)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL5 antibody [SAA0374] {PerCP} (STJA0029980)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL5 antibody [SAA0374] {PE} (STJA0030946)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Western blot analysis of various lysates using IL5RA Rabbit pAb (STJ111288) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-IL5RA antibody (20-335) (STJ111288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of K562 cells using IL-5R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL5RA antibody (41-90 aa) (STJ96579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HUVEC cells, using IL6 Rabbit monoclonal antibody (STJ11104756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-IL6 antibody (1-212) [S4756RM] (STJ11104756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, IL-6 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL6 antibody (131-180 aa) (STJ96713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit polyclonal antibody (STJ110936) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL6 antibody (30-212) (STJ110936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IL6 using IL6 Rabbit polyclonal antibody (STJ113711) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IL6 antibody (30-212) (STJ113711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IL6 antibody (Val30-Met212) [10C6] (STJA0018413)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£197.50 - £275.50

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Anti-IL6 antibody (Val30-Met212) [10G10] (STJA0018412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£197.50 - £275.50

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Western blot analysis using IL-6 mouse mAb against IL-6 recombinant protein.

Anti-IL6 antibody [6D9A1/6C12B1] (STJ98174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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