• Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit polyclonal antibody (STJ27616) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of IL4 in paraffin-embedded human liver damage using IL4 Rabbit polyclonal antibody (STJ27616) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IL4 using IL4 Rabbit polyclonal antibody (STJ27616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-IL4 antibody (25-153) (STJ27616)

SKU:
STJ27616

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL4 (25-153) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL4
Gene ID: 3565
Uniprot ID: IL4_HUMAN
Immunogen Region: 25-153
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-153 of human IL4 (NP_000580.1).
Immunogen Sequence: HKCDITLQEIIKTLNSLTEQ KTLCTELTVTDIFAASKNTT EKETFCRAATVLRQFYSHHE KDTRCLGATAQQFHRHKQLI RFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIM REKYSKCSS
Function Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6.
Protein Name Interleukin-4
Il-4
B-Cell Stimulatory Factor 1
Bsf-1
Binetrakin
Lymphocyte Stimulatory Factor 1
Pitrakinra
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-9012546
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-4 antibody
Anti-Il-4 antibody
Anti-B-Cell Stimulatory Factor 1 antibody
Anti-Bsf-1 antibody
Anti-Binetrakin antibody
Anti-Lymphocyte Stimulatory Factor 1 antibody
Anti-Pitrakinra antibody
Anti-IL4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance