Immune System | Research Area | St John's Laboratory

Immunohistochemistry analysis of IL-1 alpha in paraffin-embedded human lung cancer using IL-1 alpha Rabbit polyclonal antibody (STJ113557) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-IL1A antibody (113-267) (STJ113557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-IL1A antibody (8-57 aa) (STJ93686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using IL-1 Alpha monoclonal antibody against truncated IL-1 Alpha recombinant protein.

Anti-IL1A antibody [1F3B3/3A6F11] (STJ98170)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-IL1A/IL1F1 antibody [SAA0366] (STJA0027545)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {APC} (STJA0028946)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {FITC} (STJA0027980)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {PerCP} (STJA0029912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {PE} (STJA0030878)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL1A/IL1F1 antibody [SAA0367] (STJA0027546)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {APC} (STJA0028947)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {FITC} (STJA0027981)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {PerCP} (STJA0029913)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {PE} (STJA0030879)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ24174) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-IL1B antibody (1-269) (STJ24174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-IL-1β (PTR2541) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Untreated THP-1 Lane 2: THP-1 treated with LPS (100ng/ml) for 3hPredicted band size: 31, 18kDaObserved band size: 31, 18kDa

Anti-IL1B antibody (150-250) [PTR2541] (STJA0006256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, IL-1 Beta Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL1B antibody (181-230 aa) (STJ96566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from THP-1 cells, using IL1 Beta Rabbit polyclonal antibody (STJ118720) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IL1B antibody (70-269) (STJ118720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-IL1B/IL1F2 antibody [SAA0368] (STJA0027547)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL1B/IL1F2 antibody [SAA0368] {APC} (STJA0028948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL1B/IL1F2 antibody [SAA0368] {FITC} (STJA0027982)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL1B/IL1F2 antibody [SAA0368] {PerCP} (STJA0029914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL1B/IL1F2 antibody [SAA0368] {PE} (STJA0030880)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL1B/IL1F2 antibody [SAA0369] (STJA0027548)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL1B/IL1F2 antibody [SAA0369] {APC} (STJA0028949)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL1B/IL1F2 antibody [SAA0369] {FITC} (STJA0027983)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL1B/IL1F2 antibody [SAA0369] {PerCP} (STJA0029915)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL1B/IL1F2 antibody [SAA0369] {PE} (STJA0030881)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Western blot analysis of mouse kidney, mouse heart, mouse brain, mouse spleen and rat spleen using IL1F10 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-IL1F10 antibody (101-150 aa) (STJ97678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using CD121a antibody (STJ11104863) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1R1 antibody (21-332) (STJ11104863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of MCF7 cells using IL-1RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R1 antibody (31-80 aa) (STJ96628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse liver, using IL1R1 Rabbit polyclonal antibody (STJ28294) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IL1R1 antibody (380-569) (STJ28294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of PC12 cells using IL-1RII Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R2 antibody (141-190 aa) (STJ96835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse lung using IL1R2 Rabbit polyclonal antibody (STJ26184) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-IL1R2 antibody (20-320) (STJ26184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-IL1RAPL1 antibody (340-420 aa) (STJ192329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using IL1RL1 Rabbit polyclonal antibody (STJ11100593) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.

Anti-IL1RL1 antibody (19-328) (STJ11100593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-IL1RL1 antibody (251-300 aa) (STJ98813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-IL1RL1/ST2 antibody (Lys19-Phe328) [1A048] (STJA0021751)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£197.50 - £275.50

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Anti-IL1RL1/ST2 antibody (Lys19-Phe328) [1A049] (STJA0021752)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£197.50 - £275.50

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Anti-IL1RL1/ST2 antibody [11A11] (STJA0027009)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£315.50

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Anti-IL1RL1/ST2 antibody [11A11] {APC} (STJA0029502)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£391.50

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Anti-IL1RL1/ST2 antibody [11A11] {FITC} (STJA0028536)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£315.50

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Anti-IL1RL1/ST2 antibody [11A11] {PerCP} (STJA0030468)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£365.50

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Anti-IL1RL1/ST2 antibody [11A11] {PE} (STJA0031434)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£391.50

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Anti-IL1RL1/ST2 antibody [C2244] (STJA0018424)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-IL1RL1/ST2 antibody [CNTO3914] (STJA0018423)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-IL1RL1/ST2 antibody [SAA0139] (STJA0027008)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£315.50

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Anti-IL1RL1/ST2 antibody [SAA0139] {APC} (STJA0029503)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£391.50

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Anti-IL1RL1/ST2 antibody [SAA0139] {FITC} (STJA0028537)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£315.50

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