Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Phospho-AKT1 antibody western blot MCF-7 cells STJA0036180

Anti-Phospho-AKT1-Ser124 antibody [R04-4X5] (STJA0036180)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of various cells using Phospho-Akt1 (S129) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser129 antibody (95-144 aa) (STJ90484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-AKT1-Ser129 antibody (STJ99594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Phospho-AKT1 S129 antibody western blot MCF-7 STJA0036168

Anti-Phospho-AKT1-Ser129 antibody [R05-1C4] (STJA0036168)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser246 antibody (212-261 aa) (STJ90165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HeLa cells, using Phospho-AKT1-T308 Rabbit pAb (STJ11103747) at 1:1000 dilution. HeLa cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ11103747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-AKT1-T308 Rabbit pAb (STJ22013) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-AKT1-T308 antibody (STJ22013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 Rabbit pAb (STJ22008) at 1:600 dilution. RD cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AKT1-T450 antibody (STJ22008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Anti-Phospho-AKT1-Thr-34 antibody (STJA0005253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rabbit/Rat

$504.39

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Phospho-AKT1 Thr450 antibody western blot U2OS C6 STJA0035050

Anti-Phospho-AKT1-Thr450 antibody [R07-4A7] (STJA0035050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Phospho-AKT1 antibody western blot 3T3 lysates STJA0035669

Anti-Phospho-AKT1-Thr450 antibody [R72-1U-9] (STJA0035669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit polyclonal antibody (STJ11105868) at 1:900 dilution. 293F cells, 293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT2-S474 antibody (STJ11105868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$198.03 - $853.53

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Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Phospho-AMPK alpha 1 antibody western blot Ramos STJA0035051

Anti-Phospho-AMPK alpha 1-Ser496 antibody [R01-3B7] (STJA0035051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Phospho-AMPK alpha 2 antibody western blot 293T STJA0036159

Anti-Phospho-AMPK alpha 2-Ser345 antibody [R04-5T2] (STJA0036159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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AMPK beta 1 antibody western blot 293T cells STJA0036166

Anti-Phospho-AMPK alpha 2-Ser491 antibody [R05-9Q9] (STJA0036166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Phospho-AMPK alpha antibody western blot mouse heart STJA0035991

Anti-Phospho-AMPK alpha 2-Thr172 antibody [R03-6K2] (STJA0035991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunohistochemistry analysis of paraffin-embedded human brain, using APC1 (Phospho-Ser355) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ANAPC1-Ser355 antibody (321-370 aa) (STJ91228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ANAPC1-Ser688 antibody (654-703 aa) (STJ91312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Androgen Receptor antibody (550-600) (STJ506200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-Phospho-Androgen Receptor antibody (550-600) {Biotin} (STJ506201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Anti-Phospho-Androgen Receptor antibody (550-600) {FITC} (STJ506202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor (Phospho-Ser213) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Ser213 antibody (186-235 aa) (STJ90179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western Blot analysis of 1 HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Ser308 antibody (STJA0010044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor (Phospho-Ser650) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Ser650 antibody (621-670 aa) (STJ90180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Ser81 antibody (STJA0010072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells, using Androgen Receptor (Phospho-Ser94) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Ser94 antibody (66-115 aa) (STJ91328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot of HeLa lysate showing specific immunolabeling of the ~110 kDa AR protein phosphorylated at Ser94 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-AR-Ser94 antibody (STJA0003579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

$674.13

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Tyr267 antibody (STJA0010079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using Androgen Receptor (Phospho-Tyr363) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Tyr363 antibody (331-380 aa) (STJ90599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Tyr534 antibody (STJA0010084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Argonaute 2-Ser-387 antibody (STJA0041078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Argonaute 2-Tyr-393 antibody (STJA0041079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Western blot analysis of various cells using Phospho-ATF-2 (S112) Polyclonal Antibody diluted at 1：500

Anti-Phospho-ATF2-Ser112 antibody (79-128 aa) (STJ90183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human brain, using ATF-2 (Phospho-Ser472) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Ser472 antibody (441-490 aa) (STJ91240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATF2 (Phospho-Ser480) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Ser480 antibody (456-505 aa) (STJ91069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

$67.62 - $360.18

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Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.

Anti-Phospho-ATF2-Ser490/498 antibody (STJA0003583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

$674.13

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Western blot analysis of various cells using Phospho-ATF-2 (S62) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ATF2-Ser62 antibody (29-78 aa) (STJ90184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T69/71 Rabbit pAb (STJ22702). 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATF2-T69/71 antibody (STJ11101048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from 293T cells, using Phospho-ATF2-T69/T71 Rabbit pAb (STJ110861) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit

Anti-Phospho-ATF2-T69/T71 antibody (STJ110861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

Choose Options