Gene Expression And Transcription | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-NOC2L antibody (602-651) (STJ94525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from A-549 cells, using Non-phospho-YAP1-S127 Rabbit pAb (STJ11103239) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Non-phospho-YAP1-S127 antibody (50-150) (STJ11103239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using NOP2 antibody (STJ11103028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NOP2 antibody (355-590) (STJ11103028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-NOP2 antibody (STJA0007573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$67.62 - $360.18

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Western blot analysis of extracts of Rat brain cells, using Notch1 antibody (STJ29951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (2456-2555) (STJ29951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g Notch1 antibody (STJ11101627). Western blot was performed from the immunoprecipitate using Notch1 antibody (STJ11101627) at a dilution of 1:1000.

Anti-NOTCH1 antibody (2456-2555) [S7MR] (STJ11101627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of extracts of U-251MG cells, using Notch1 antibody (STJ119090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-NOTCH1 antibody (2504-2518) (STJ119090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-Notch1 antibody [PT0229R] (STJA0039691)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-NOTCH1 antibody [SAA0131] (STJA0026984)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$351.21 - $522.33

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Anti-NOTCH1 antibody [SAA0131] {APC} (STJA0029442)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$428.49 - $652.05

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Anti-NOTCH1 antibody [SAA0131] {FITC} (STJA0028476)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$351.21 - $522.33

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Anti-NOTCH1 antibody [SAA0131] {PerCP} (STJA0030408)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$403.65 - $607.89

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Anti-NOTCH1 antibody [SAA0131] {PE} (STJA0031374)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$428.49 - $652.05

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Anti-NOTCH1 nanobody [SAA1286] (STJN000228)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$522.33 - $2,252.85

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Immunofluorescence analysis of C6 cells using NOTCH2 Rabbit polyclonal antibody (STJ11105517) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH2 antibody (1697-1819) (STJ11105517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of L929 cells using NOTCH2 antibody (STJ29808) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH2 antibody (2242-2471) (STJ29808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-Notch2 antibody [PT0367R] (STJA0039531)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-NOTCH2 nanobody [SAA1287] (STJN000257)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$522.33 - $2,252.85

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Immunofluorescence analysis of U2OS cells using NOTCH3 Rabbit polyclonal antibody (STJ24789) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH3 antibody (1520-1640) (STJ24789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-NOTCH3 antibody (2222-2321 aa) [SRM5292] (STJ11107194)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Immunohistochemistry analysis of paraffin-embedded mouse pancreas using NOTCH3 antibody (STJ115483) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NOTCH3 antibody (2305-2321) (STJ115483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of L929 cells using NOTCH3 Rabbit polyclonal antibody (STJ24788) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH3 antibody (751-850) (STJ24788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Anti-NOTCH3 antibody (C-Term) (STJA0005815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human

$628.59

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Anti-NOTCH3 antibody [PT1351R] (STJA0040049)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Rat

$207.00 - $745.20

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Anti-NOTCH3 nanobody [SAA1288] (STJN000313)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$522.33 - $2,252.85

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Western blot analysis of lysates from Mouse lung, using NOTCH4 Rabbit pAb (STJ110601) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NOTCH4 antibody (1824-2003) (STJ110601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-NOTCH4 antibody [SAA1712] (STJA0020140)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$471.27 - $1,997.55

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NPAS4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NPAS4 antibody (603-652) (STJ94544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-NPAS4 antibody (STJ501951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$589.95

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Anti-NPAS4 antibody {Biotin} (STJ501952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-NPAS4 antibody {FITC} (STJ501953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Nucleophosmin antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-NPM1 antibody (1-19 aa) [R06-4G7] (STJA0011706)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$340.17

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Western blot analysis of various cells using Nucleophosmin Polyclonal Antibody diluted at 1：2000

Anti-NPM1 antibody (1-50) (STJ94573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Nucleophosmin (ABT210) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 33kDaObserved band size: 33kDa

Anti-NPM1 antibody (100-200 aa) [ABT210] (STJ197178)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

$248.40 - $360.18

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Western blot analysis of various cells using NPM Polyclonal Antibody

Anti-NPM1 antibody (201-250) (STJ94546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of U2OS cells using NPM1 Rabbit polyclonal antibody (STJ119961) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NPM1 antibody (9-122) (STJ119961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis using B23 monoclonal antibody against HeLa, Jurkat, 3T3 cell lysate.

Anti-NPM1 antibody (STJ98470)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Pig/Rabbit

$205.62 - $496.80

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Western blot analysis using B23 monoclonal antibody against SMMC-7721 (1) , HepG2 (2) , HeLa (3) and HEK293 (4) cell lysate.

Anti-NPM1 antibody [7H10B9] (STJ97858)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

$205.62 - $496.80

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Confocal imaging of paraffin-embedded mouse heart using NPPA Rabbit monoclonal antibody (STJ11104694, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-NPPA antibody (1-100) [S4694RM] (STJ11104694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.57 - $765.21

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Immunohistochemistry analysis of paraffin-embedded rat heart using NPPA Rabbit polyclonal antibody (STJ116955) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NPPA antibody (26-151) (STJ116955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using NPPA Rabbit polyclonal antibody (STJ24798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NPPA antibody (26-151) (STJ24798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NPPA antibody (STJ192988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-NPY antibody (20-70 aa) (STJ13101441)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat/Rabbit/Xenopus

$457.47

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Anti-NPY antibody (20-70 aa) (STJ13101442)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat/Rabbit/Xenopus

$457.47

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Western blot analysis of extracts of SH-SY5Y cells, using NPY antibody (STJ24802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NPY antibody (29-97) (STJ24802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of NPY antibody in paraffin-embedded human brain tissue.

Anti-NPY antibody (45-94) (STJ94547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-NPY antibody [SAA0521] (STJA0019356)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

$522.33 - $2,252.85

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Anti-NPY/Neuropeptide Y antibody [L115/13] (STJA0019718)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human

$522.33 - $2,252.85

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