• Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ24145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (81-259) (STJ24145)

SKU:
STJ24145

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IGFBP1 (81-259) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP1
Gene ID: 3484
Uniprot ID: IBP1_HUMAN
Immunogen Region: 81-259
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 81-259 of human IGFBP1 (NP_000587.1).
Immunogen Sequence: GLSCRALPGEQQPLHALTRG QGACVQESDASAPHAAEAGS PESPESTEITEEELLDNFHL MAPSEEDHSILWDAISTYDG SKALHVTNIKKWKEPCRIEL YRVVESLAKAQETSGEEISK FYLPNCNKNGFYHSRQCETS MDGEAGLCWCVYPWNGKRIP GSPEIRGDPNCQIYFNVQN
Post Translational Modifications Phosphorylated.probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Protein Name Insulin-Like Growth Factor-Binding Protein 1
Ibp-1
Igf-Binding Protein 1
Igfbp-1
Placental Protein 12
Pp12
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 1 antibody
Anti-Ibp-1 antibody
Anti-Igf-Binding Protein 1 antibody
Anti-Igfbp-1 antibody
Anti-Placental Protein 12 antibody
Anti-Pp12 antibody
Anti-IGFBP1 antibody
Anti-IBP1 antibody

Information sourced from Uniprot.org

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