Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-DNMT3B antibody (1-50 aa) (STJ92753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Chicken

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells using DNMT3B Rabbit polyclonal antibody (STJ23415) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DNMT3B antibody (345-853) (STJ23415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Dnmt3b antibody [R09-7G6] (STJA0035734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-DNMT3B antibody [SRM14995] (STJ11109725)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ChIP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using DNMT3L antibody (STJ23417) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DNMT3L antibody (100-200) (STJ23417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using DNMT3L antibody (STJ115552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DNMT3L antibody (151-386) (STJ115552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-DNMT3L antibody (52-102) (STJA0006683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Dnmt3L antibody [R06-8Z-6] (STJA0033140)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

£214.50 - £308.50

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Anti-DP1 antibody [R03-1T2] (STJA0037322)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various cells using DPF2 Polyclonal Antibody

Anti-DPF2 antibody (151-200 aa) (STJ92773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using DPF2 Rabbit polyclonal antibody (STJ11100215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-DPF2 antibody (90-200) (STJ11100215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-DPF2 antibody [R39-2M-4] (STJA0035571)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-DPF3 antibody (45-95) (STJA0006372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of Mouse heart, using DPF3 antibody (STJ119219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DPF3 antibody (90-200) (STJ119219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-DPY30 antibody (39-89 aa) (STJ196596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Mouse testis, using DR1 antibody (STJ28983) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DR1 antibody (1-176) (STJ28983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-DR1 antibody (STJA0008252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-DR4 antibody [SRM13417] (STJ11108147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-DR5 antibody (STJA0037906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Anti-DR5 antibody [DR5-01] (STJ16100937)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£560.50

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Immunofluorescence analysis of HeLa cells using DROSHA antibody (STJ11101722). Blue: DAPI for nuclear staining.

Anti-DROSHA antibody (1-100) [S2MR] (STJ11101722)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of SH-SY5Y K562 HELA using RNase III Drosha antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DROSHA antibody (774-823 aa) (STJ97614)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Drosha antibody [PT0944R] (STJA0039819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Drosha antibody [R01-9I2] (STJA0036421)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-DYRK2 antibody (320-400 aa) (STJ190110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of A-549 cells using DYRK2 antibody (STJ29092). Blue: DAPI for nuclear staining.

Anti-DYRK2 antibody (429-528) (STJ29092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of DYRK2 in paraffin-embedded Human kidney tissue using DYRK2 Rabbit polyclonal antibody (STJ11105335) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-DYRK2 antibody (500-600) (STJ11105335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Immunohistochemistry with Anti-DYRK2 Antibody (STJ500807) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-DYRK2 antibody (550-601) (STJ500807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-DYRK2 antibody (550-601) {Biotin} (STJ500808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-DYRK2 antibody (550-601) {FITC} (STJ500809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-DYRK2 antibody (STJA0010272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-E2-EPF/UBE2S antibody (1-222aa) [AT2C12] (STJA0041579)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-E2F1 antibody (100-170 aa) (STJ98642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-E2F1 antibody (125-165) (STJ500811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£438.50

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Anti-E2F1 antibody (125-165) {Biotin} (STJ500815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-E2F1 antibody (125-165) {FITC} (STJ500816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

Choose Options

Immunofluorescence analysis of C6 cells using E2F1 Polyclonal Antibody (STJ23457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-E2F1 antibody (200-301) (STJ23457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using E2F1 Rabbit polyclonal antibody (STJ119131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-E2F1 antibody (301-437) (STJ119131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-E2F1 antibody (338-437) [S9MR] (STJ11101709)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-E2F1 antibody (380-430) (STJ500812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£438.50

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Anti-E2F1 antibody (380-430) {Biotin} (STJ500813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

Choose Options

Anti-E2F1 antibody (380-430) {FITC} (STJ500814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot with Anti-E2F1 Antibody (STJ500810) with peptide on at 1:500 dilution in buffer.

Anti-E2F1 antibody (58-93) (STJ500810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£438.50

Choose Options

Anti-E2F1 antibody (58-93) {Biotin} (STJ500817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

Choose Options

Anti-E2F1 antibody (58-93) {FITC} (STJ500818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot detection of E2F-1 in C6, Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-E2F1 antibody [4G8-2C2-C12] (STJ99250)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Rat

£149.00 - £360.00

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Anti-E2F1 antibody [4G8-2C2-C12] (STJA0032477)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-E2F1 antibody [R03-5G3] (STJA0034260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options