Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.

Anti-SOX2 antibody [2A10-F11-F12] (STJ99043)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ICC

Reactivity :

Mouse

£144.00 - £348.00

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Anti-SOX9 antibody (1-100 aa) (STJ11107028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-SOX9 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 56 kDaObserved band size: 70 kDa

Anti-SOX9 antibody (1-100 aa) [ABT-SOX9] (STJ196900)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Sox-9 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SOX9 antibody (147-196 aa) (STJ95736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using SOX9 antibody (STJ11101779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SOX9 antibody (169-300) [S9MR] (STJ11101779)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of HepG2 cells, using SOX9 antibody (STJ111160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-SOX9 antibody (250-339) (STJ111160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using SP1 Rabbit polyclonal antibody (STJ116866) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SP1 antibody (420-620) (STJ116866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ Hep G2 cells with 1 Mu g SP1 rabbit monoclonal antibody (STJ11101745) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2) , as shown in figure.

Anti-SP1 antibody (500-600) [S5MR] (STJ11101745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of SP1 in paraffin-embedded Human small intestine tissue using SP1 Rabbit pAb (STJ11107080) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-SP1 antibody (581-680 aa) (STJ11107080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Sp1 Polyclonal Antibody

Anti-SP1 antibody (706-755 aa) (STJ95737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SP7 antibody (280-360 aa) (STJ190436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of Rat brain, using SP7 antibody (STJ11101490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SP7 antibody (333-376) (STJ11101490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using SPI1 Rabbit polyclonal antibody (STJ11103316) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SPI1 antibody (17-98) (STJ11103316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using PU.1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SPI1 antibody (181-230 aa) (STJ95271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from Rat kidney, using Osteopontin Rabbit polyclonal antibody (STJ25680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SPP1 antibody (106-205) (STJ25680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using Osteopontin antibody (STJ25679) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SPP1 antibody (17-314) (STJ25679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Osteopontin antibody (STJ11101629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SPP1 antibody (200-300) [S9MR] (STJ11101629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various cells using OPN Polyclonal Antibody diluted at 1：500

Anti-SPP1 antibody (261-310 aa) (STJ94832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Osteopontin antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-SPP1 antibody (287-300 aa) [R08-1C5] (STJA0012170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Hamster/Rat

£264.50

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Anti-SPP1 antibody (N-Term) [M574] (STJA0005500)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various lysates using Osteopontin Mouse monoclonal antibody (STJ11104172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPP1 antibody [S4172MM] (STJ11104172)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Src antibody (STJ11101656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SRC antibody (1-100) [S6MR] (STJ11101656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Src Rabbit polyclonal antibody (STJ113306) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SRC antibody (1-80) (STJ113306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SRC antibody (200-300) (STJ503200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SRC antibody (200-300) {Biotin} (STJ503202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SRC antibody (200-300) {FITC} (STJ503203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of rat-lung tissue. 1, c-Src Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-SRC antibody (360-440 aa) (STJ92501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-SRC primary antibody (STJ503201) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SRC antibody (400-500) (STJ503201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SRC antibody (400-500) {Biotin} (STJ503204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SRC antibody (400-500) {FITC} (STJ503205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Src (PTR2316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HUVEC Lane 3: A431Predicted band size: 60kDaObserved band size: 60kDa

Anti-SRC antibody (400-536 aa) [PTR2316] (STJ193243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, using Src Rabbit polyclonal antibody (STJ25689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SRC antibody (410-536) (STJ25689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using Src polyclonal antibody (STJ11100197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRC antibody (430-530) (STJ11100197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SRC antibody (N-Term) [M259] (STJA0005601)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Src mouse Monoclonal antibody diluted at 1:200.

Anti-SRC antibody [Mix] (STJ196656)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunofluorescence analysis of C6 cells using SREBF1 Rabbit polyclonal antibody (STJ118039) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SREBF1 antibody (200-310) (STJ118039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 1, mouse-liver 2, hela 3, mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-SREBF1 antibody (250-330 aa) (STJ98832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

£48.00 - £252.00

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Anti-SREBF1 antibody (950-1000) (STJ503128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SREBF1 antibody (950-1000) {Biotin} (STJ503130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SREBF1 antibody (950-1000) {FITC} (STJ503131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates using SRF Rabbit polyclonal antibody (STJ119129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SRF antibody (1-280) (STJ119129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of A-431, using SRF antibody (STJ11100944) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SRF antibody (321-420) (STJ11100944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using SRF Polyclonal Antibody

Anti-SRF antibody (71-120 aa) (STJ95779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SRF antibody (STJ13100381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Immunohistochemistry analysis of SRRM1 in paraffin-embedded mouse testis using SRRM1 Rabbit polyclonal antibody (STJ27860) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SRRM1 antibody (1-160) (STJ27860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western Blot analysis of Hela lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-SRRM1 antibody (STJ191765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SRRT antibody (403-453 aa) (STJ195542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Rat liver using SRRT Rabbit pAb (STJ11100951) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.

Anti-SRRT antibody (623-872) (STJ11100951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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