• Western blot analysis of extracts of various cell lines, using Phospho-KRAS-S89 rabbit polyclonal antibody (STJ11102656) at 1:500 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of Phospho-KRAS-S89 in paraffin-embedded mouse kidney using Phospho-KRAS-S89 Rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Phospho-KRAS-S89 rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-KRAS-S89 in paraffin-embedded rat brain using Phospho-KRAS-S89 Rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-KRAS-S89 rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Phospho-KRAS-S89 Rabbit polyclonal antibody (STJ11102656) at 1:500 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-KRAS-S89 antibody (STJ11102656)

SKU:
STJ11102656

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-KRAS-S89 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRAS
Gene ID: 3845
Uniprot ID: RASK_HUMAN
Immunogen: A synthetic phosphorylated peptide around S89 of human KRAS (NP_001356715.1).
Immunogen Sequence: TKSFE
Post Translational Modifications Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180. Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.
Protein Name Gtpase Kras
K-Ras 2
Ki-Ras
C-K-Ras
C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed
Database Links Reactome: R-HSA-112412
Reactome: R-HSA-1169092
Reactome: R-HSA-1236382
Reactome: R-HSA-1250196
Reactome: R-HSA-1250347
Reactome: R-HSA-1433557
Reactome: R-HSA-167044
Reactome: R-HSA-171007
Reactome: R-HSA-179812
Reactome: R-HSA-180336
Reactome: R-HSA-186763
Reactome: R-HSA-1963640
Reactome: R-HSA-210993
Reactome: R-HSA-2179392
Reactome: R-HSA-2424491
Reactome: R-HSA-2428933
Reactome: R-HSA-2871796
Reactome: R-HSA-375165
Reactome: R-HSA-4086398 P01116-2
Reactome: R-HSA-442982
Reactome: R-HSA-5218921
Reactome: R-HSA-5621575
Reactome: R-HSA-5637810
Reactome: R-HSA-5654688
Reactome: R-HSA-5654693
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5658442
Reactome: R-HSA-5673000
Reactome: R-HSA-5673001
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802953
Reactome: R-HSA-6802955
Reactome: R-HSA-74751
Reactome: R-HSA-8849471
Reactome: R-HSA-8851805
Reactome: R-HSA-8951936
Reactome: R-HSA-9026519
Reactome: R-HSA-9027284
Reactome: R-HSA-9028731
Reactome: R-HSA-9034864
Reactome: R-HSA-9607240
Reactome: R-HSA-9634285
Reactome: R-HSA-9634635
Reactome: R-HSA-9648002
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9703465
Reactome: R-HSA-9703648
Reactome: R-HSA-9753510
Reactome: R-HSA-9753512
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Endomembrane System
Cytoplasm
Cytosol
Isoform 2b: Cell Membrane
Alternative Antibody Names Anti-Gtpase Kras antibody
Anti-K-Ras 2 antibody
Anti-Ki-Ras antibody
Anti-C-K-Ras antibody
Anti-C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed antibody
Anti-KRAS antibody
Anti-KRAS2 antibody
Anti-RASK2 antibody

Information sourced from Uniprot.org

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