• Western blot analysis of extracts from HT29 cells, using Phospho-JunB-S259 antibody (STJ22215). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of lysates from HT29 cells, using Phospho-JunB-S259 Rabbit polyclonal antibody (STJ22215). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.
  • Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-JunB-S259 antibody (STJ22215).

Anti-Phospho-JUNB-S259 antibody (STJ22215)

SKU:
STJ22215

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-JUNB-S259 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUNB
Gene ID: 3726
Uniprot ID: JUNB_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S259 of human Phospho-JunB-S259
Immunogen Sequence: Email for sequence
Post Translational Modifications Ubiquitinated by ITCH, leading to its degradation.
Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGAGCTCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity.
Protein Name Transcription Factor Junb
Transcription Factor Ap-1 Subunit Junb
Database Links Reactome: R-HSA-2173796
Reactome: R-HSA-6785807
Reactome: R-HSA-9031628
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Junb antibody
Anti-Transcription Factor Ap-1 Subunit Junb antibody
Anti-JUNB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance