Anti-Phospho-LIPE-Ser855 antibody (520-569 aa) (STJ90762)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human HSL around the phosphorylation site of Ser855/554 at the amino acid range 520-569
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Hormone-sensitive lipase-Ser855 (520-569 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LIPE |
| Gene ID | 3991 |
| Uniprot ID | LIPS_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human HSL around the phosphorylation site of Ser855/554 at the amino acid range 520-569 |
| Immunogen Region | 520-569 aa |
| Specificity | Phospho-HSL (S855) Polyclonal Antibody detects endogenous levels of HSL protein only when phosphorylated at S855. |
Additional Info
| Post Translational Modifications | Phosphorylation by AMPK reduces its translocation towards the lipid droplets. |
| Function | Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. |
| Protein Name | Hormone-Sensitive LipaseHslMonoacylglycerol Lipase LipeRetinyl Ester HydrolaseReh |
| Database Links | Reactome: R-HSA-163560Reactome: R-HSA-9841922 |
| Cellular Localisation | Cell MembraneMembraneCaveolaCytoplasmCytosolLipid DropletFound In The High-Density CaveolaeTranslocates To The Cytoplasm From The Caveolae Upon Insulin StimulationPhosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets |
| Alternative Antibody Names | Anti-Hormone-Sensitive Lipase antibodyAnti-Hsl antibodyAnti-Monoacylglycerol Lipase Lipe antibodyAnti-Retinyl Ester Hydrolase antibodyAnti-Reh antibodyAnti-LIPE antibody |
Information sourced from Uniprot.org