• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAPKAPK5 (Phospho-Thr182) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-MAPKAPK5-Thr182 antibody (148-197 aa) (STJ90980)

SKU:
STJ90980

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MAP kinase-activated protein kinase 5-Thr182 (148-197 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPKAPK5
Gene ID: 8550
Uniprot ID: MAPK5_HUMAN
Immunogen Region: 148-197 aa
Specificity: Phospho-PRAK (T182) Polyclonal Antibody detects endogenous levels of PRAK protein only when phosphorylated at T182.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MAPKAPK5 around the phosphorylation site of Thr182 at the amino acid range 148-197
Post Translational Modifications Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4.which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated.
Function Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Protein Name Map Kinase-Activated Protein Kinase 5
Mapk-Activated Protein Kinase 5
Mapkap Kinase 5
Mapkap-K5
Mapkapk-5
Mk-5
Mk5
P38-Regulated/Activated Protein Kinase
Prak
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-5687128
Reactome: R-HSA-6804756
Cellular Localisation Cytoplasm
Nucleus
Translocates To The Cytoplasm Following Phosphorylation And Activation
Interaction With Erk3/Mapk6 Or Erk4/Mapk4 And Phosphorylation At Thr-182
Activates The Protein Kinase Activity
Followed By Translocation To The Cytoplasm
Phosphorylation By Pka/Prkaca At Ser-115 Also Induces Nuclear Export
Alternative Antibody Names Anti-Map Kinase-Activated Protein Kinase 5 antibody
Anti-Mapk-Activated Protein Kinase 5 antibody
Anti-Mapkap Kinase 5 antibody
Anti-Mapkap-K5 antibody
Anti-Mapkapk-5 antibody
Anti-Mk-5 antibody
Anti-Mk5 antibody
Anti-P38-Regulated/Activated Protein Kinase antibody
Anti-Prak antibody
Anti-MAPKAPK5 antibody
Anti-PRAK antibody

Information sourced from Uniprot.org

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