Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using Phospho-HER2/ErbB2-Y1221/1222 Rabbit pAb (STJ110863) at 1:1000 dilution. HeLa cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-ERBB2-Y1221/1222 antibody (STJ110863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of A-431, using Phospho-ErbB2 (Y1221 + Y1222) Rabbit monoclonal antibody (STJ11105879) at 1:1000 dilution. A-431 cells were treated by EGF (200 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-ERBB2-Y1221/Y1222 antibody [S5879RM] (STJ11105879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Western blot A431 cells ERK1-2 phosphorylation detection with calyculin A and EGF treatment STJA0041190

Anti-Phospho-ERK1, ERK2-Thr-202/Tyr-204 antibody [M206] (STJA0041190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£579.50

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Phospho-ERK1/2 antibody western blot NIH/3T3 lysates STJA0035674

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R49-1Y-4] (STJA0035674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-ESR Alpha-S106 Rabbit pAb (STJ22134). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S106 antibody (STJ22134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-ESR Alpha-S118 pAb (STJ22133) at 1:1000 dilution. MCF7 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ESR1-S118 antibody (STJ22133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ESR Alpha-S118 Rabbit mAb (STJ11102593) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ESR1-S118 antibody [S3MR] (STJ11102593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£143.50 - £618.50

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Western blot analysis of lysates from MCF7 cells using Phospho-ESR Alpha-S167 Rabbit pAb (STJ22135). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S167 antibody (STJ22135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser102) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser102 antibody (71-120 aa) (STJ91254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-ER Alpha (S104) Polyclonal Antibody

Anti-Phospho-ESR1-Ser104 antibody (71-120 aa) (STJ90262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of RAT-BRAIN cells using Phospho-ER Alpha (S106) Polyclonal Antibody diluted at 1：500

Anti-Phospho-ESR1-Ser106 antibody (71-120 aa) (STJ90263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-ER Alpha (S118) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-ESR1-Ser118 antibody (91-140 aa) (STJ90264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser167) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser167 antibody (136-185 aa) (STJ90265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using Estrogen Receptor-alpha (Phospho-Ser305) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser305 antibody (276-325 aa) (STJ91336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of 1 HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ESR1-Thr311 antibody (STJA0010024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-ER Alpha (Y537) Polyclonal Antibody

Anti-Phospho-ESR1-Tyr537 antibody (501-550 aa) (STJ90808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-ER alpha S118 antibody western blot MCF-7 STJA0036000

Anti-Phospho-Estrogen Receptor alpha-Ser118 antibody [R03-3T5] (STJA0036000)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Estrogen Receptor Alpha-Tyr-537 antibody (STJA0041221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Anti-Phospho-Estrogen Receptor Alpha-Tyr-537 antibody [M545] (STJA0041199)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Western blot analysis of lysates from C6 cells, using Phospho-EZH2/KMT6-T311 Rabbit pAb (STJ11105805) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-EZH2-T311 antibody (STJ11105805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£126.50 - £618.50

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Anti-Phospho-EZH2-Thr311 antibody (STJ196302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from MDA cells, using Phospho-Ezrin-T567 Rabbit pAb (STJ22136). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-EZR-T567 antibody (STJ22136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HELA COLO using Phospho-Ezrin (T566) antibody. Antibody was diluted at 1:1000

Anti-Phospho-EZR-Thr566 antibody (534-583 aa) (STJ90266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Ezrin (Y146) Polyclonal Antibody

Anti-Phospho-EZR-Tyr146 antibody (111-160 aa) (STJ90569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells treated with EGF 200ng/ml 30', using Ezrin (Phospho-Tyr353) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EZR-Tyr353 antibody (321-370 aa) (STJ90267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Ezrin (Phospho-Tyr478) Antibody. The lane on the right was blocked with the Ezrin STJ90570

Anti-Phospho-EZR-Tyr478 antibody (446-495 aa) (STJ90570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, using Phospho-Ezrin-Y353 Rabbit pAb (STJ29814). 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-EZR-Y353 antibody (STJ11101152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Phospho-Ezrin/Radixin/Moesin antibody western blot 3T3 cells STJA0035065

Anti-Phospho-Ezrin/Radixin/Moesin-Thr567/Thr564/Thr558 antibody [R03-1G3] (STJA0035065)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat/Hamster

£133.50 - £281.50

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Western blot FAK and phospho-FAK Tyr-397 detection in HUVEC lysates STJA0041226

Anti-Phospho-FAK-Tyr-397 antibody [M121] (STJA0041226)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat/Rabbit

£542.50

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Phospho-FAK Y397 antibody western blot 293 cells STJA0035977

Anti-Phospho-FAK-Tyr397 antibody [R01-3L2] (STJA0035977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-FAK Tyr397 antibody western blot C6 lysates STJA0033930

Anti-Phospho-FAK-Tyr397 antibody [R05-1A1] (STJA0033930)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat

£133.50 - £281.50

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Phospho-FAK Tyr576 antibody western blot A549 U251 C2C12 STJA0035066

Anti-Phospho-FAK-Tyr576 antibody [R08-3A7] (STJA0035066)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-FAK-Y576/Y577 antibody [SRM13711] (STJ11108441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western Blot analysis of 1 HepG2 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FES-Tyr713 antibody (STJA0009935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-FGFR1 antibody (640-690) (STJ502423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£497.50

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Anti-Phospho-FGFR1 antibody (640-690) {Biotin} (STJ502424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£551.50

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Anti-Phospho-FGFR1 antibody (640-690) {FITC} (STJ502425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£551.50

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Western blot analysis of various cells using Phospho-Flg (Y154) Polyclonal Antibody

Anti-Phospho-FGFR1-Tyr154 antibody (121-170 aa) (STJ90678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-FGFR1-Tyr653+Tyr654 antibody (STJA0009929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FGFR1-Tyr653/654 antibody (STJ196303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of Hela cells using Phospho-Flg (Y654) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-FGFR1-Tyr654 antibody (626-675 aa) (STJ90688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of SY-SY5Y using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-FGFR1-Tyr730/733 antibody (STJA0010039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FGFR1 (Phospho-Tyr766) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FGFR1-Tyr766 antibody (736-785 aa) (STJ90810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, using Phospho-FGFR1-Y653 pAb (STJ22141) at 1:1000 dilution. 293T cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-FGFR1-Y653 antibody (STJ22141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Phospho-FGFR1-Y653/Y654 antibody (STJ11105816) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-FGFR1-Y653/Y654 antibody (STJ11105816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-FOXO1-S319 Rabbit pAb (STJ11101126) at 1:1000 dilution or FOXO1 antibody (STJ115801). HeLa cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-FOXO1-S319 antibody (STJ11101126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western Blot analysis of HepG2 mouse brain tissue, rat brain tissue, using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-FOXO1-Ser249 antibody (STJA0009891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-FOXO1-Ser256 antibody (223-272 aa) (STJ90273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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