| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Ezrin-Tyr478 (446-495 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EZR |
| Gene ID: |
7430 |
| Uniprot ID: |
EZRI_HUMAN |
| Immunogen Region: |
446-495 aa |
| Specificity: |
Phospho-Ezrin (Y478) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at Y478. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Ezrin around the phosphorylation site of Tyr478 at the amino acid range 446-495 |
| Post Translational Modifications | Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. |
| Protein Name | EzrinCytovillinVillin-2P81 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-437239Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | Apical Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionMicrovillus MembraneRuffle MembraneCytoplasmCell CortexCytoskeletonMicrovillusLocalization To The Apical Membrane Of Parietal Cells Depends On The Interaction With Pals1Localizes To Cell Extensions And Peripheral Processes Of AstrocytesMicrovillar Peripheral Membrane Protein (Cytoplasmic Side) |
| Alternative Antibody Names | Anti-Ezrin antibodyAnti-Cytovillin antibodyAnti-Villin-2 antibodyAnti-P81 antibodyAnti-EZR antibodyAnti-VIL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
