Phospho Antibodies | St John's Laboratory

Western blot analysis of various lysates using Phospho-MAP3K5-S83 Rabbit pAb (STJ11100961) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour, treated by TNF-Alpha (20ng/mL) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP3K5-S83 antibody (STJ11100961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of lysates from Jurkat cells, using Phospho-MAP3K5-S967 Rabbit pAb (STJ11103750) at 1:1000 dilution. Jurkat cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MAP3K5-S967 antibody (STJ11103750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of 293 cells using Phospho-ASK 1 (S83) Polyclonal Antibody

Anti-Phospho-MAP3K5-Ser83 antibody (49-98 aa) (STJ90181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-ASK 1 (S966) Polyclonal Antibody

Anti-Phospho-MAP3K5-Ser966 antibody (932-981 aa) (STJ90182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from 293T cells, using Phospho-MAP3K5-T845 Rabbit pAb (STJ11103748) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAP3K5-T845 antibody (STJ11103748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of lysates from HeLa cells, using Phospho-MAP3K5-T918 Rabbit pAb (STJ22251) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP3K5-T918 antibody (STJ22251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-MAP3K5-Thr838 antibody (STJA0009839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-MAP3K5-Thr838 antibody (STJA0009885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-MAP3K5-Thr845 antibody (STJ196244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using Phospho-TAK1-S412 Rabbit pAb (STJ22254) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 120s.

Anti-Phospho-MAP3K7-S412 antibody (STJ22254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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NLRC5 Deficiency Reduces LPS-Induced Microglial Activation via Inhibition of NF-κB Signaling and Ameliorates Mice’s Depressive-like Behavior. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Jianqiong Zhang WB mouse 1:500 BV2 cell

Anti-Phospho-MAP3K7-Ser412 antibody (STJ196446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293T using p-Tak1 (S439) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP3K7-Ser439 antibody (411-460 aa) (STJ90947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-MAP3K7-T187 antibody (STJ117909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using TAK1 (Phospho-Thr184) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP3K7-Thr184 antibody (161-210 aa) (STJ90538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293T cells using Phospho-Tak1 (T187) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP3K7-Thr187 antibody (161-210 aa) (STJ90584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K8 (Phospho-Ser400) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAP3K8-Ser400 antibody (366-415 aa) (STJ91209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Cot (T290) Polyclonal Antibody

Anti-Phospho-MAP3K8-Thr290 antibody (256-305 aa) (STJ91027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-MAP4-Ser696 antibody (662-711 aa) (STJ91318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-MAPK1/2-Thr-188 antibody (STJA0005413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (STJA0003631, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.

Anti-Phospho-MAPK1/2-Thr202/Tyr204 antibody (STJA0003631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Xenopus/Zebrafish

$613.58

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (STJ110835) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody (STJ110835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (STJ11102568) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (STJ11102154) at1:3000 dilution (lower) incubated overnight at 4 °C. 293T cells were treated by PMA (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 10s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody [S8MR] (STJ11102568)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western Blot analysis of 1, HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAPK12-Tyr185/182 antibody (STJA0009932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from C6 cells, using Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (STJ11103773) at 1:500 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK14 & MAPK11 & MAPK13 & MAPK12-T180/Y182 & T180/Y182 & T180/Y182 & T183/Y1845 antibody (STJ11103773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-p38 MAPK-Y182 Rabbit polyclonal antibody (STJ11105887) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180 antibody (STJ11105887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (STJ110862) at 1:18000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-MAPK14-T180/Y182 antibody (STJ110862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of Phospho-p38 MAPK-T180/Y182 in paraffin-embedded Mouse kidney using Phospho-p38 MAPK-T180/Y182 Rabbit polyclonal antibody (STJ11105812) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180/Y182 antibody (STJ11105812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of C6, using Phospho-p38 MAPK-T180/Y182 Rabbit monoclonal antibody (STJ11105903) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 ug/ml) at 37 °C for 20 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-MAPK14-T180/Y182 antibody [S5903RM] (STJ11105903)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Mapk14-Thr-180/Tyr-182 antibody [M139] (STJA0005505)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$532.58

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180 antibody (147-196 aa) (STJ90362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

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Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0 NA or 30 NA showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.

Anti-Phospho-MAPK14-Thr180/Tyr182 antibody (STJA0003734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Bovine/Canine/Chicken/Non-Human Primates/Rat/Zebrafish

$613.58

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180/Y182 antibody (151-200 aa) (STJ90498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/FC/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Guinea Pig

$64.80 - $340.20

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Anti-Phospho-Mapk14-Tyr-323 antibody (STJA0005506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Western blot analysis of lysates from 1) PC-3, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-MAPK14-Tyr182 antibody (147-196 aa) (STJ90363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of human-stomach tissue. 1, p38 (phospho Tyr323) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-MAPK14-Tyr323 antibody (288-337 aa) (STJ90878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (STJ22257) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-MAPK14-Y182 antibody (STJ22257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using Phospho-p38 MAPK-T180/Y182 antibody (STJ11102653) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-MAPK14/MAPK11/MAPK13/MAPK12-T180/Y182 antibody (STJ11102653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

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Western blot analysis of Jurkat cells using Phospho-ERK 8 (T175/Y177) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MAPK15-Thr175/Y177 antibody (141-190 aa) (STJ90517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of various lysates using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (STJ22261) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-T202 antibody (STJ22261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 + ERK2-Y187 Rabbit pAb (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from 293 cells, using Phospho-ERK3-S189 Rabbit pAb (STJ22263) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAPK6-S189 antibody (STJ22263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ERK3 (Phospho-Ser189) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAPK6-Ser189 antibody (155-204 aa) (STJ90898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-MAPK8-Thr-183/Tyr-185 antibody [M268] (STJA0005456)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Western blot analysis of various lysates, using Phospho-JNK1/2-T183/Y185 Rabbit pAb (STJ110836) at 1:5000 dilution. 293T, NIH/3T3 and C6 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK8/MAPK9-T183/Y185 antibody (STJ110836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (STJ113512) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK8/MAPK9/MAPK10-T183/T183/T221 antibody [S2MR] (STJ113512)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various lysates using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb (STJ11102651) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK8/MAPK9/MAPK10-T183/TY185 & T183/Y185 & T221/Y223 antibody (STJ11102651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of lysates from C6 cells untreated or treated with anisomycin using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb (STJ22264). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAPK8/MAPK9/MAPK10-T183/Y185 & T183/Y185 & T221/Y223 antibody (STJ22264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of COLO cells using Phospho-JIP-1 (T103) Polyclonal Antibody

Anti-Phospho-MAPK8IP1-Thr103 antibody (69-118 aa) (STJ90715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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