Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$197.78 - $867.38

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Anti-Phospho-AKT1-Thr-34 antibody (STJA0005253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rabbit/Rat

$493.43

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Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-AKT1-Thr342 antibody (STJA0003576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

D.melanogaster

$613.58

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Akt1/3 (Y437/434) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-Akt1/3-Tyr437/434 antibody (406-455 aa) (STJ90938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from C6 cells using Phospho-Akt-S473 Rabbit pAb (STJ11101106) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-AKT1/AKT2/AKT3-S473/S474/S472 antibody (STJ11101106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb (STJ11102660) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-AKT1/AKT2/AKT3-T308/T309/T305 antibody (STJ11102660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-AKT1S1-Ser183 antibody (STJ194735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from HeLa cells, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (STJ117891) at 1:400 dilution. HeLa cells were treated by Insulin (100 nM) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 45s.

Anti-Phospho-AKT1S1-T246 antibody (STJ117891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AKT1S1-Thr246 antibody (207-256 aa) (STJ90462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot of Drosophila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-AKT1S1-Thr356 antibody (STJA0003754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

D.melanogaster

$613.58

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Western blot analysis of lysates from HeLa cells using Phospho-AKT1S1/PRAS40-T246 Rabbit mAb (STJ11107731) at 1:8100 dilution incubated overnight at 4C. HeLa cells were treated by Insulin (50 nM) at 37C for 5 minutes after serum-starvation overnight. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3% nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 60s.

Anti-Phospho-AKT1S1/PRAS40-T246 antibody [SRM5542] (STJ11107731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit polyclonal antibody (STJ11105868) at 1:900 dilution. 293F cells, 293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT2-S474 antibody (STJ11105868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ALK-Tyr1078 antibody (STJ196236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using ALK (Phospho-Tyr1096) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ALK-Tyr1096 antibody (1062-1111 aa) (STJ91196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-Phospho-ALK-Tyr1278 antibody (STJ196237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-ALK-Tyr1278/1282/1283 antibody (STJ196238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30', using ALK (Phospho-Tyr1507) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ALK-Tyr1507 antibody (1473-1522 aa) (STJ90845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Anti-Phospho-ALK-Tyr1586 antibody (STJ193212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ALK-Tyr1604 antibody (1570-1619 aa) (STJ91058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from HL-60 and HL-60 cells, using Phospho-ALK-Y1096 Rabbit pAb (STJ110843) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight. HL-60 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ALK-Y1096 antibody (STJ110843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of lysates from HT-29 cells, using Phospho-ALOX5-S271 Rabbit pAb (STJ29339) at 1:1000 dilution. HT-29 cells were treated by PMA/TPA (200nM). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ALOX5-S271 antibody (STJ29339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody

Anti-Phospho-ALOX5-Ser272 antibody (246-295 aa) (STJ90875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-ALOX5-Ser523 antibody (STJ90651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$64.80 - $340.20

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Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-ALOX5-Ser523 antibody (STJA0003568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Non-Human Primates

$613.58

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Western Blot analysis of mouse brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ALOX5-Ser663 antibody (STJA0010042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-alpha Actinin 4-S159 antibody (140-190) (STJ502086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Anti-Phospho-alpha Actinin 4-S159 antibody (140-190) {Biotin} (STJ502087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$789.08

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Anti-Phospho-alpha Actinin 4-S159 antibody (140-190) {FITC} (STJ502088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$789.08

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Dot-blot analysis of all sorts of peptides using Phospho-alt-RPL36-S140 antibody (STJ11105799) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-alt-RPL36-S140 antibody (STJ11105799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Dot-blot analysis of all sorts of peptides using Phospho-alt-RPL36-S142 antibody (STJ11105800) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-alt-RPL36-S142 antibody (STJ11105800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Dot-blot analysis of all sorts of peptides using Phospho-alt-RPL36-S19 antibody (STJ11105797) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-alt-RPL36-S19 antibody (STJ11105797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Dot-blot analysis of all sorts of peptides using Phospho-alt-RPL36-S22 antibody (STJ11105798) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-alt-RPL36-S22 antibody (STJ11105798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-AMPK Alpha 1-Ser485 antibody [PT1374R] (STJA0040378)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of lysates from C2C12 cells, using Phospho-AMPK Alpha 1-T183/AMPK Alpha 2-T172 Rabbit pAb (STJ11107614) at 1:1000 dilution. C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AMPK Alpha 1-T183 + T172 antibody (STJ11107614)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of Hela cell. 1, AMPK Alpha 1/2 (phospho Thr183/172) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-AMPK Alpha 1/2-Thr183/172 antibody (140-189 aa) (STJ90735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Pig/M.japonicus

$64.80 - $340.20

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Anti-Phospho-AMPK Alpha 1/2-Thr183/Thr172 antibody [PT0920R] (STJA0039809)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of lysates from C2C12 cells, using Phospho-AMPK Beta 1-S182 Rabbit pAb (STJ11103734) at 1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-AMPK Beta 1-S182 antibody (STJ11103734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183 + AMPKa2-T172 Rabbit monoclonal antibody (STJ11105834) at1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-AMPKa1 + AMPKa2-T183-T172 antibody [S5834RM] (STJ11105834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$197.78 - $867.38

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Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit monoclonal antibody (STJ11105892) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-AMPKalpha1+AMPKalpha2-T183-T172 antibody [S5892RM] (STJ11105892)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human brain, using APC1 (Phospho-Ser355) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ANAPC1-Ser355 antibody (321-370 aa) (STJ91228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ANAPC1-Ser688 antibody (654-703 aa) (STJ91312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from NIH-3T3 cells, using Phospho-Annexin I (Y21) Polyclonal Antibody.

Anti-Phospho-ANXA1-Tyr21 antibody (STJ91361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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