Anti-Phospho-ALOX5-Ser663 antibody (STJA0010042)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human ALOX5 (Phospho Ser663)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Polyunsaturated fatty acid 5-lipoxygenase-Ser663 for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALOX5 |
| Gene ID | 240 |
| Uniprot ID | LOX5_HUMAN |
| Immunogen | Synthesized peptide derived from human ALOX5 (Phospho Ser663) |
| Specificity | This antibody detects endogenous levels of ALOX5 (Phospho Ser663) Rabbit pAb at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-524 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. Phosphorylation at Ser-524 is stimulated by 8-bromo-3',5'-cyclic AMP or prostaglandin E2. |
| Function | Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells. |
| Protein Name | Polyunsaturated Fatty Acid 5-LipoxygenaseArachidonate 5-Lipoxygenase5-Lo5-Lipoxygenase |
| Database Links | Reactome: R-HSA-2142688Reactome: R-HSA-2142691Reactome: R-HSA-2142700Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-9012546Reactome: R-HSA-9018676Reactome: R-HSA-9018682Reactome: R-HSA-9018896Reactome: R-HSA-9020265Reactome: R-HSA-9023661Reactome: R-HSA-9026286Reactome: R-HSA-9026290Reactome: R-HSA-9026403Reactome: R-HSA-9027604 |
| Cellular Localisation | CytoplasmNucleus MatrixNucleus MembranePeripheral Membrane ProteinPerinuclear RegionCytosolNucleus EnvelopeNucleus Intermembrane SpaceShuttles Between Cytoplasm And NucleusFound Exclusively In The NucleusWhen Phosphorylated On Ser-272Calcium Binding Promotes Translocation From The Cytosol And The Nuclear Matrix To The Nuclear Envelope And Membrane Association |
| Alternative Antibody Names | Anti-Polyunsaturated Fatty Acid 5-Lipoxygenase antibodyAnti-Arachidonate 5-Lipoxygenase antibodyAnti-5-Lo antibodyAnti-5-Lipoxygenase antibodyAnti-ALOX5 antibodyAnti-LOG5 antibody |
Information sourced from Uniprot.org