Anti-Phospho-AKT1S1-Thr356 antibody (STJA0003754)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-PRAS40-Thr356 for use in WB in D.melanogaster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | D.melanogaster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 conjugated to KLH. |
Additional Info
| Background | PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt-and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012). |
Information sourced from Uniprot.org