Post Translational Modifications | Primary antibodies

Phospho-ErbB2 antibody western blot A431 cells STJA0035681

Anti-Phospho-ErbB2-Tyr1139 antibody [R78-8J-4] (STJA0035681)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-ERBB2-Tyr1196 antibody (STJ196320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Polyclonal Antibody

Anti-Phospho-ERBB2-Tyr1221/Y1222 antibody (1191-1240 aa) (STJ90337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-ERBB2-Tyr1221/Y1222 antibody (STJ91025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Neu (Y1248) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ERBB2-Tyr1248 antibody (1206-1255 aa) (STJ90338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Neu (Y877) Polyclonal Antibody diluted at 1：500

Anti-Phospho-ERBB2-Tyr877 antibody (851-900 aa) (STJ90339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A-431 cells, using Phospho-HER2/ErbB2-Y1139 (STJ11103785) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ErbB2-Y1139 antibody [S5MR] (STJ11103785)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HER2/ErbB2-Y1221/1222 Rabbit pAb (STJ110863) at 1:1000 dilution. HeLa cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-ERBB2-Y1221/1222 antibody (STJ110863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of A-431, using Phospho-ErbB2 (Y1221 + Y1222) Rabbit monoclonal antibody (STJ11105879) at 1:1000 dilution. A-431 cells were treated by EGF (200 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-ERBB2-Y1221/Y1222 antibody [S5879RM] (STJ11105879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Western blot analysis of 293T cells using Phospho-ErbB-3 (Y1197) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ERBB3-Tyr1197 antibody (1163-1212 aa) (STJ90642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ERBB3-Tyr1222 antibody (1191-1240 aa) (STJ91083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-ErbB3 antibody western blot SKBR3 cells STJA0035676

Anti-Phospho-ErbB3-Tyr1222 antibody [R53-7S-8] (STJA0035676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of Hela cells using Phospho-ErbB-3 (Y1289) Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ERBB3-Tyr1289 antibody (1256-1305 aa) (STJ91342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ERBB3-Tyr1328 antibody (1293-1342 aa) (STJ91148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (STJ11102622) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ERBB3-Y1222 antibody [S2MR] (STJ11102622)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of extracts from MCF7 cells, using Phospho-ErbB3/HER3-Y1289 Rabbit monoclonal antibody (STJ11105873) at 1:1000 dilution. MCF7 cells were treated by neuregulin at 37 °C for 15 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-Phospho-ERBB3-Y1289 antibody [S5873RM] (STJ11105873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from A-431 cells, using Phospho-ErbB3/HER3-Y1328 Rabbit pAb (STJ22130) at 1:2000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-ERBB3-Y1328 antibody (STJ22130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western Blot analysis of mouse brain tissue, MCF-7 rat brain tissue using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-ERBB4-Tyr1242 antibody (STJA0010023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ERBB4-Tyr1284 antibody (1250-1299 aa) (STJ91084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-ERBB4-Tyr984 antibody (STJ196321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from MCF7 cells using Phospho-ErbB4/HER4-Y1284 Rabbit pAb (STJ22131) at 1:700 dilution. MCF7 cells were treated by IGF (200 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 20 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-ERBB4-Y1284 antibody (STJ22131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-ErbB4/HER4-Y1284 antibody [SRM13706] (STJ11108436)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various cells using Phospho-Erbin (Y1104) Polyclonal Antibody

Anti-Phospho-ERBIN-Tyr1104 antibody (STJ90259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ERF (Phospho-Thr526) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ERF-Thr526 antibody (492-541 aa) (STJ91289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of mouse-liver tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202 antibody (169-218 aa) (STJ90260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Erk 1/2-Thr202/Tyr204 antibody [PT0643R] (STJA0039299)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of rat-lung tissue. 1, ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202/Y204 antibody (STJ91357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish

£49.00 - £261.00

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Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Tyr204 antibody (170-219 aa) (STJ90261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-tubulin monoclonal antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-ERK-Tyr222/205 antibody (STJ90657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot (WB): The picture was kindly provided by our customer. The primary antibody was diluted at 1:2000. The STJ97738

Anti-Phospho-ERK-Y222/205 antibody (140-220 aa) [4G3] (STJ97738)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot A431 cells ERK2 phosphorylation with calyculin A and phosphatase treatment STJA0041209

Anti-Phospho-ERK1 (T207) /ERK2 (T188)-Thr-188 antibody (STJA0041209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101064)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101067)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Western blot A431 cells ERK1-2 phosphorylation detection with calyculin A and EGF treatment STJA0041190

Anti-Phospho-ERK1, ERK2-Thr-202/Tyr-204 antibody [M206] (STJA0041190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£579.50

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Phospho-ERK1/2 antibody western blot HeLa TPA lysates STJA0033905

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R05-2H9] (STJA0033905)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

£133.50 - £281.50

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Phospho-ERK1/2 antibody western blot HeLa C6 cells STJA0033913

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R07-3G4] (STJA0033913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Phospho-ERK1/2 antibody western blot NIH/3T3 lysates STJA0035674

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R49-1Y-4] (STJA0035674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb (201245, 1:500 diluted, upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246, 1:500 diluted, lower).

Anti-Phospho-ERK1/2-Thr202/Tyr204 antibody [2E8-2F6-C12] (STJ99286)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

£149.00 - £360.00

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phospho-ERK1/2 antibody western blot A431 lysates EGF STJA0035749

Anti-Phospho-ERK1/2-Thr202/Tyr204+Thr185/Tyr187 antibody [R08-8S3] (STJA0035749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Phospho-ERK1/2 antibody immunohistochemistry human colon carcinoma STJA0032931

Anti-Phospho-ERK1/2-Tyr222/Tyr205 antibody [10C8-9F6-10C8] (STJA0032931)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Phospho-ERK1/2 antibody immunohistochemistry human colon carcinoma STJA0032903

Anti-Phospho-ERK1/2-Tyr222/Tyr205 antibody [1H4-6D7-9D8] (STJA0032903)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Phospho-ERK1/2 antibody western blot C6 HeLa lysates STJA0034288

Anti-Phospho-ERK1/2/-Thr202/Tyr204+Thr185/Tyr187 antibody [R04-9E9] (STJA0034288)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ERK1/ERK2-T202/T185 antibody [S6MR] (STJ11102556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IRE1-S724 Rabbit pAb (STJ11105788) at 1:800 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-ERN 1-S724 antibody (STJ11105788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-Separase (S801) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ESPL1-Ser801 antibody (767-816 aa) (STJ91032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from MCF7 cells, using Phospho-ESR Alpha-S106 Rabbit pAb (STJ22134). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S106 antibody (STJ22134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

£126.50 - £618.50

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