• Western blot analysis of extracts of A-431 cells, using Phospho-ErbB3/HER3-Y1328 antibody (STJ22130) at 1:2000 dilution. A-431 cells were treated by Serum-starvation overnight at 37 °C. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of A-431 cells, using Phospho-ErbB3/HER3-Y1328 antibody (STJ22130) at 1:2000 dilution. A-431 cells were treated by Serum-starvation overnight at 37 °C. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-ERBB3-Y1328 antibody (STJ22130)

SKU:
STJ22130

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ERBB3-Y1328 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERBB3
Gene ID: 2065
Uniprot ID: ERBB3_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y1328 of human ERBB3 (NP_001973.2).
Immunogen Sequence: PDYWH
Tissue Specificity Epithelial tissues and brain.
Post Translational Modifications Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
Function Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it.ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation.
Protein Name Receptor Tyrosine-Protein Kinase Erbb-3
Proto-Oncogene-Like Protein C-Erbb-3
Tyrosine Kinase-Type Cell Surface Receptor Her3
Database Links Reactome: R-HSA-1227986 P21860-1
Reactome: R-HSA-1236394 P21860-1
Reactome: R-HSA-1250196 P21860-1
Reactome: R-HSA-1257604 P21860-1
Reactome: R-HSA-1306955 P21860-1
Reactome: R-HSA-1358803 P21860-1
Reactome: R-HSA-1963642 P21860-1
Reactome: R-HSA-2219530 P21860-1
Reactome: R-HSA-5673001 P21860-1
Reactome: R-HSA-6785631 P21860-1
Reactome: R-HSA-6811558 P21860-1
Reactome: R-HSA-8847993 P21860-1
Reactome: R-HSA-8863795 P21860-1
Reactome: R-HSA-9664565 P21860-1
Reactome: R-HSA-9665686 P21860-1
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Receptor Tyrosine-Protein Kinase Erbb-3 antibody
Anti-Proto-Oncogene-Like Protein C-Erbb-3 antibody
Anti-Tyrosine Kinase-Type Cell Surface Receptor Her3 antibody
Anti-ERBB3 antibody
Anti-HER3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance