Post Translational Modifications | Primary antibodies

Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H4-R3 Rabbit pAb (STJ11106422) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MonoMethyl-Histone H4-R3 antibody (STJ11106422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€151.80 - €742.20

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MonoMethyl-p53 antibody immunohistochemistry human lung carcinoma STJA0032959

Anti-MonoMethyl-p53-Lys370 antibody [4C7-7E1-8E2] (STJA0032959)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

€160.20 - €337.80

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Immunofluorescence analysis of HeLa cells using N5-Methylcytidine Rabbit polyclonal antibody (STJ29880) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-N5-Methyl-m5C antibody (STJ29880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€132.60 - €742.20

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The m6A mouse monoclonal antibody (STJ11100594) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer). Oligomer 8- N6-methyladenosine (ATAACTGG-m6A-CCGAATGG) Oligomer 9- unmodified adenosine (ATAACTGGACCGAATGG) Oligomer 10- unmodified adenosine (AAAAAAAAAAAAAAAA-biotin)

Anti-N6-Methyl-m6A antibody [S4MM] (STJ11100594)

Clonality :

Monoclonal

Host :

Mouse

Applications :

DB/ELISA

Reactivity :

Species Independent

€142.20 - €625.80

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RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA, using 5 µg of the N6-methyladenosine/ m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR. The picture shows the relative enrichment multiple of HS6ST1 site.

Anti-N6-Methyl-m6A antibody [S4MR] (STJ11101784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

DB/IF/ICC/ELISA/meRIP

Reactivity :

Species Independent

€172.20 - €742.20

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Anti-Pan-Acetyl-ACTA2-Lys52/50/51 antibody (STJA0005951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Pan-Acetyl-ACTA2-Lys63/61/62 antibody (STJA0006005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Pan-Acetyl-KRT2-Lys185 antibody (STJA0005971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using Pan Acetyl-Lysine Mouse mAb (STJ11106167) at 1:1000 dilution. HeLa、NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Pan-Acetyl-Lysine antibody [SMM5007] (STJ11106167)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€172.20 - €742.20

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Pan-Acetyl-MYH1-Lys1394 antibody (STJA0005982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Pan-Acetyl-MYH1-Lys505 antibody (STJA0005983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ11100252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Pan-Di-Methyl-Kme2 antibody (STJ11100252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ27698) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Pan-Di-Methyl-Lysine antibody (STJ27698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€151.80 - €742.20

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Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (STJ11100217) at 1:1000 dilution.

Anti-Pan-Di-Methyl-Rme2s antibody (STJ11100217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€151.80 - €742.20

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Dot-blot analysis of all sorts using pan-methyl-Histidine polyclonal antibody (STJ11103884) at 1:1000 dilution. Peptide 1：Peptide library (3-methylhistidine-intermediate) Peptide 2：Peptide library (3-methylhistidine-C-terminal) Peptide 3：Peptide library (1-methylhistidine-intermediate) Peptide 4：Peptide library (1-methylhistidine-C-terminal) Peptide 5：Histidine methylation modified peptide library mixPeptide 6：Control peptide library mix

Anti-pan-Methyl-Histidine antibody (STJ11103884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Pan-Methyl-Lysine antibody (STJ197343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species independent

€58.80 - €313.20

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Western blot analysis of Hela using Pan Methylated Lysine monoclonal antibody.

Anti-Pan-Methyl-Lysine-Mix antibody [Mix] (STJ97446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Species independent

€58.80 - €313.20

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Western blot analysis of various lysates using pan-Mono-Methyl R Motif Rabbit polyclonal antibody (STJ11103886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-pan-Mono-Methyl-Arginine Motif antibody (STJ11103886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody (STJ11100249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Pan-Mono-Methyl-Kme1 antibody (STJ11100249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of extracts of various cell lines, using pan-Mono-MethylR (R

Anti-Pan-Mono-Methyl-Rme1 antibody (STJ11100253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-Serine/Threonine Rabbit pAb (STJ11101030) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 45s.

Anti-Pan-Phospho-p-S/T antibody (STJ11101030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-PKC (pan) (gamma) -T514 Rabbit pAb (STJ29379) at 1:1000 dilution. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Pan-Phospho-PKC Gamma-T514 antibody (STJ29379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Pan-Phospho-PKC-Beta II-S660 antibody [SRM13709] (STJ11108439)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Anti-Pan-Phospho-Serine antibody [SAA0307] (STJA0019180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

Broad-Spectrum

€234.60 - €1,583.40

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Western blot analysis of various lysates using Phospho- (Ser/Thr) ATM/ATR Substrate pAb (STJ11101067) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Pan-Phospho-Serine/Threonine antibody (STJ11101067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from A-431 cells using pan Phospho-Serine/Threonine Mouse mAb (STJ11101105) at 1:1000 dilution incubated overnight at 4 °C. A-431 cells were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/> Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.

Anti-Pan-Phospho-Serine/Threonine antibody [S5MM] (STJ11101105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

€172.20 - €742.20

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Anti-Pan-Phospho-Serine/Threonine antibody [SAA0308] (STJA0019181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

Broad-Spectrum

€234.60 - €1,583.40

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Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit polyclonal antibody (STJ11105908) at 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Phospho-T antibody (STJ11105908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Other

€151.80 - €742.20

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine pAb (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody (STJ11101040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€151.80 - €742.20

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Anti-Pan-Phospho-Tyrosine antibody [4G10] (STJA0018700)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Broad-Spectrum

€234.60 - €1,583.40

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Anti-Pan-Phospho-Tyrosine antibody [PY20] (STJA0019177)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Broad-Spectrum

€234.60 - €1,583.40

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Western blot analysis of various lysates using Pan Phospho-Tyrosine Rabbit mAb (STJ11102650) at 1:1000 dilution. Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Pan-Phospho-Tyrosine antibody [S0MR] (STJ11102650)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

€172.20 - €742.20

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine Mouse mAb (STJ11101091) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody [S1MM] (STJ11101091)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€142.20 - €625.80

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Anti-Pan-Phospho-Tyrosine antibody [SAA0305] (STJA0019178)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Broad-Spectrum

€234.60 - €1,583.40

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Anti-Pan-Phospho-Tyrosine antibody [SAA0306] (STJA0019179)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Broad-Spectrum

€234.60 - €1,583.40

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Anti-Pan-Phospho-Tyrosine antibody [SRM15116] (STJ11109846)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

€142.20 - €625.80

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Western blot analysis of various lysates, using Pan-SymmetricDi-MethylArginineMotif Rabbit monoclonal antibody (STJ11103452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Symmetric Di-Methyl-Arginine Motif antibody [S2MR] (STJ11103452)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€142.20 - €625.80

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Western blot analysis of extracts of various cell lines, using Pan-Symmetric Di-Methyl Arginine Motif antibody (STJ11104843) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Pan-Symmetric Di-Methyl-Arginine Motif antibody [S4843RM] (STJ11104843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€172.20 - €742.20

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Western blot analysis of various lysates using pan-TriMethyl-lysine polyclonal antibody (STJ11100248) at 1:500 dilution. HeLa and MCF7 cells were treated by ADOX (100 Mu M) for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Pan-Tri-Methyl-Kme3 antibody (STJ11100248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using pan-Tri-Methyl lysine antibody (STJ11103178) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Pan-Tri-Methyl-Lysine antibody (STJ11103178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

€151.80 - €742.20

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Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-14-3-3 Beta/Zeta-Ser184/186 antibody (151-200 aa) (STJ90562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-14-3-3-Ser58 antibody (STJA0003567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Sheep/Xenopus/Zebrafish

€586.20

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Anti-Phospho-4E BP1-Thr46 antibody [R03-5C7] (STJA0033917)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

€168.60 - €370.20

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Anti-Phospho-4E-BP1 )-Thr37/46 antibody [PT0862R] (STJA0039150)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-4E-BP1-Thr69 antibody (35-84 aa) (STJ90163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-4E-BP1-Thr70 antibody [PT1203R] (STJA0040179)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of lysates from COS7 cells treated with EGF 200 ng/ml for 30', using ABL1 (Phospho-Thr735) STJ90579

Anti-Phospho-ABL1-Thr735 antibody (701-750 aa) (STJ90579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Anti-Phospho-ABL1-Thr735 antibody (STJ196256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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