Monoclonal Antibodies

Rabbit Monoclonal Antibodies

Hybridoma-derived antibodies from a larger starting B cell pool

Our rabbit monoclonal antibodies are generated by immunising rabbits and fusing antibody-producing B cells with a myeloma line to create a stable hybridoma clone. A rabbit spleen contains many more B cells than a mouse spleen, so each fusion starts from a larger pool of candidate antibodies, giving a greater chance of finding the right clone for a given target.

How it works

Hybridoma production from a larger B cell pool

Rabbits are immunised with the target antigen, and B cells from the immunised animal are fused with a myeloma cell line to form a hybridoma, which is cultured to produce a single, traceable antibody clone. The main advantage of using a rabbit over a mouse for this process is the starting material: a rabbit spleen holds far more B cells than a mouse spleen. A larger starting pool means more candidate antibodies to screen, which raises the odds of identifying a clone with the binding properties needed for a given target.

Category benefits

Why use rabbit monoclonal antibodies?

✓ A wider pool of candidate clones
Because a rabbit spleen yields more B cells than a mouse spleen, each fusion generates more candidate antibodies to screen against your target.
✓ Broad reactivity, including pathogen targets
Clones in the range are validated against human, mouse and rat targets, alongside pathogen-specific clones such as our SARS-CoV-2 nucleocapsid and spike antibodies.
✓ A single, traceable epitope
As with all our monoclonal antibodies, each rabbit clone descends from one parent hybridoma cell, giving consistent, repeatable binding to the same epitope across experiments.
✓ A wide range of conjugates for panel design
Our rabbit clones are available across ABflo® fluorescent conjugates spanning the visible to near-infrared spectrum, giving flexibility when building multicolour panels.
Where they're used

Typical applications

✓ Western blotting and ELISA
Many clones in the range are validated for western blot and ELISA, supporting detection and quantification of target proteins in lysates and plate-based assays.
✓ IHC, immunofluorescence and ICC
Selected clones are validated for IHC, IF and ICC, localising target proteins within tissue sections and fixed cells.
✓ Flow cytometry and immunoprecipitation
Certain clones are validated for flow cytometry (FC) and immunoprecipitation (IP), supporting cell population analysis and target pull-down from lysates.
✓ Chromatin immunoprecipitation
Selected clones are validated for ChIP, supporting studies of protein-DNA interactions in chromatin research.

Need help choosing a rabbit monoclonal antibody?

Contact our technical support team for help selecting a clone and application format for your target of interest. Each product page includes a downloadable datasheet and SDS, with dispatch on overnight priority shipping with gel ice packs.

Looking for more monoclonal antibodies?

Browse our full range of monoclonal antibodies across other host species.

 View all monoclonal antibodies

For Research Use Only. Not for use in diagnostic procedures.

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