Anti-CD27 antibody [PT1331R] (STJA0040116)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgGk
STJA0040116
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General Information

Short DescriptionRabbit monoclonal anti-Cd27 antigen for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDPT1331R
IsotypeIgGk
ConjugationUnconjugated
PurificationProtein A
Dilution RangeIHC 1:200-1:1000
WB 1:10000-1:50000
IF 1:200-1:1000
ELISA 1:5000-1:20000
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCD27
Gene ID939
Uniprot IDCD27_HUMAN
SpecificityEndogenous

Additional Info

Post Translational Modifications Phosphorylated. N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue.
Protein Name Cd27 Antigen
Cd27l Receptor
T-Cell Activation Antigen Cd27
T14
Tumor Necrosis Factor Receptor Superfamily Member 7
Cd Antigen Cd27
Database Links Reactome: R-HSA-5669034
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd27 Antigen antibody
Anti-Cd27l Receptor antibody
Anti-T-Cell Activation Antigen Cd27 antibody
Anti-T14 antibody
Anti-Tumor Necrosis Factor Receptor Superfamily Member 7 antibody
Anti-Cd Antigen Cd27 antibody
Anti-CD27 antibody
Anti-TNFRSF7 antibody

Information sourced from Uniprot.org

Citations

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