Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from Rat testis, using ASB17 Rabbit polyclonal antibody (STJ11103238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ASB17 antibody (78-177) (STJ11103238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Western blot analysis of lysates from 293T cells, using ASB2 Rabbit polyclonal antibody (STJ119909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ASB2 antibody (448-587) (STJ119909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using ASB3 Rabbit pAb (STJ11106526) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ASB3 antibody (1-145 aa) (STJ11106526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASB3 antibody (30-80 aa) (STJ194590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-ASB5 antibody (STJA0008746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-ASB6 antibody (STJA0008044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-ASB8 antibody (STJA0008851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASB9 antibody (169-219 aa) (STJ194602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates, using ASC/TMS1 antibody (STJ11104876) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASC/TMS1 antibody (1-100 aa) (STJ11104876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ70108 (0. 2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ASC/TMS1 antibody (C-Term) (STJ70108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

€390.63

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Biotinylated STJ70108 (3µg/ml) staining of U937 lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ASC/TMS1, Biotinylated antibody (C-Term) {Biotin} (STJ73188)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Western blot analysis of lysates from rat pancreas, using ASCC1 Rabbit polyclonal antibody (STJ118277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASCC1 antibody (1-180) (STJ118277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCC1 antibody (289-339 aa) (STJ194445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using ASCC2 Rabbit polyclonal antibody (STJ115734) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASCC2 antibody (488-757) (STJ115734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-ASCC2 antibody (STJA0008414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates, using ASCC3 Rabbit pAb (STJ110267) at 1:800 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 50s.

Anti-ASCC3 antibody (1-111) (STJ110267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCC3 antibody (2053-2103 aa) (STJ195324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of ASCL1 antibody in paraffin-embedded human brain tissue.

Anti-ASCL1 antibody (109-158 aa) (STJ91732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using AscL1 Rabbit polyclonal antibody (STJ118012) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AscL1 antibody (150-236) (STJ118012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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STJ73536 (1µg/ml) staining of Mouse (A) and Rat (B) Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-ASCL1 antibody (79-91) (STJ73536)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Cow/Mouse/Rat/Dog

€390.63

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCL2 antibody (1-80 aa) (STJ94020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Ascl2 antibody (185-263 aa) (STJ11106594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ72471 (0. 1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Ascl3-Mouse antibody (Internal) (STJ72471)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Cow

€390.63

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASCL4 antibody (1-70) (STJ116649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-ASCL4 antibody (81-131) (STJA0006373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Asense antibody at 1:1,000 dilution; protein at 40 ng per lane; chicken polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-Asense antibody (254 aa C-Term) (STJ140181)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

D.melanogaster

€169.38 - €493.13

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Anti-ASF1A antibody (100-204) (STJ116719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of various cells using Asf1a Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ASF1A antibody (121-170 aa) (STJ91733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot with Anti-ASF1A Antibody (STJ500171) of rat muscle membranes at 1:500 dilution in buffer.

Anti-ASF1A antibody (60-120) (STJ500171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-ASF1A antibody (60-120) {Biotin} (STJ500172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-ASF1A antibody (60-120) {FITC} (STJ500173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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STJ71364 (1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A antibody (C-Term) (STJ71364)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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STJ71180 (0. 01µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A/HSPC146 antibody (Internal) (STJ71180)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Western blot analysis of lysates from K562 cells, using ASF1B Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ASF1B antibody (101-150 aa) (STJ91734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using ASF1B antibody (STJ28594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASF1B antibody (63-202) (STJ28594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-ASFV 9GL/pB119L/P14 antibody [1A021] (STJA0019897)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV A238L antibody (Met1-Val226) [1A449] (STJA0022396)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV B117L antibody [1A026] (STJA0019902)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV B117L antibody [1A027] (STJA0019903)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV B169L antibody [1A030] (STJA0019906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV B169L antibody [1A031] (STJA0019907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV E184R antibody [1A007] (STJA0019883)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV E184R antibody [1A008] (STJA0019884)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV EP84R antibody [1A024] (STJA0019900)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV EP84R antibody [1A025] (STJA0019901)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV F317L antibody [1A012] (STJA0019888)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV F778R antibody [1A019] (STJA0019895)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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Anti-ASFV F778R antibody [1A020] (STJA0019896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

€286.88 - €410.63

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