Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103690) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£201.50 - £379.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit mAb (STJ11103694) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) [S4MR] (STJ11103694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £638.50

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Immunofluorescence analysis of Hela cell. 1, DAPK3 (phospho Thr265) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Alpha-tubulin antibody (350-451 aa) [8F11] (STJ96937)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse/D.melanogaster

£49.00 - £261.00

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Anti-Alpha-Tubulin antibody (C-Term) [DM1A] (STJA0041418)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£474.50

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Western blot analysis of C. Elegans whole body lysate using mouse monoclonal antibody diluted at 1:20,000.

Anti-Alpha-tubulin antibody [Mix] (STJ196642)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

C.elegans/Human/Mouse/Rat

£180.00 - £261.00

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Western blot analysis of various lysates using Alpha-Tubulin Mouse mAb (STJ11107556) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Alpha-Tubulin antibody [SMM5491] (STJ11107556)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Alpha-Tubulin antibody [SRM15104] (STJ11109834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Alpha-Tubulin antibody [TU-01] (STJ16100473)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IP/FC

Reactivity :

Mouse/Pig/Human/Turkey/Eisenia/Paramecium/Nicotiana/Yeast/Arabidopsis

£504.50

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Anti-alpha/beta Synuclein antibody [R04-5C4] (STJA0035897)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-alpha/beta-Tubulin dimer antibody [TU-08] (STJ16103111)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/WB

Reactivity :

Pig/Human/Mouse

£504.50

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STJ71657 (0. 1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha2-antiplasmin antibody (Internal) (STJ71657)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of 293F-ALPI, using ALPI rabbit polyclonal antibody (STJ27982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALPI antibody (20-527) (STJ27982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-ALPI antibody (STJ192955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALPK1 using ALPK1 Rabbit pAb (STJ11106978) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s. <br><b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>

Anti-ALPK1 antibody (1-473 aa) (STJ11106978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using Alpha-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ALPK1 antibody (11-60 aa) (STJ96366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using ALPK1 Rabbit polyclonal antibody (STJ11103266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALPK1 antibody (340-500) (STJ11103266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-ALPK1 antibody [SRM13365] (STJ11108095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£0.00

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Anti-ALPK3 antibody (730-810 aa) (STJ190245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Human colon cancer tissue using Alkaline Phosphatase (ALPL) Rabbit polyclonal antibody (STJ22595) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.

Anti-ALPL antibody (120-260) (STJ22595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of the lysates from Jurkat cells using ALPL antibody.

Anti-ALPL antibody (201-250 aa) (STJ96048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using Alkaline Phosphatase (Alkaline Phosphatase (ALPL) ) Rabbit polyclonal antibody (STJ114272) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ALPL antibody (230-440) (STJ114272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ73461 (0. 1µg/ml) staining of Human Kidney (A) , Lung (B) and Adrenal Gland (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ALPL antibody (42-53) (STJ73461)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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Immunohistochemistry analysis of paraffin-embedded Human placenta using Placental alkaline phosphatase (PLAP) antibody (STJ11102038) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-ALPP antibody (100-200) [S8MR] (STJ11102038)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g Placental alkaline phosphatase (PLAP) antibody (STJ11104336). Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase (PLAP) antibody (STJ11104336) at a dilution of 1:1000.

Anti-ALPP antibody (260-460) (STJ11104336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-ALPP antibody [6C7G3] (STJ16103562)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human

£746.50

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Anti-ALPP antibody [PT0484R] (STJA0039473)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of 293 cells using ALPP/ALPPL2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-ALPP/ALPPL2 antibody (1-50 aa) (STJ96740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using ALPPL2 antibody (STJ28946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ALPPL2 antibody (20-280) (STJ28946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of mouse brain cells using ALS2 Rabbit polyclonal antibody (STJ29205) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALS2 antibody (260-429) (STJ29205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ALS2 antibody (390-470 aa) (STJ190540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ALS2 antibody (N-Term) (STJ13100566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat

£322.50

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Anti-ALS2 antibody (N-Term) (STJ13100567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat

£322.50

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Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALS2CL antibody (606-656 aa) (STJ193682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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STJ70325 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total NA NA NA protein per lane). Primary incubated for 1 hour. Detected by western blot NA NA NA using chemiluminescence.

Anti-ALS2CR1/NIF3L1 antibody (C-Term) (STJ70325)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse

£312.50

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Anti-ALS2CR16 antibody (STJA0010102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

0

£49.00 - £261.00

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STJ70323 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ALS2CR2/ILPIP antibody (C-Term) (STJ70323)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Dog/Cow

£312.50

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Anti-ALS2CR8 antibody [R04-5G7] (STJA0034012)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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STJ73460 (0. 1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ALT1/GPT1 antibody (391-405) (STJ73460)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-ALX1 antibody (STJA0008042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using ALX3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ALX3 antibody (171-220 aa) (STJ91572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HepG2 cells, using ALX4 Rabbit polyclonal antibody (STJ116188) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALX4 antibody (1-220) (STJ116188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Aly antibody [R01-2A4] (STJA0034013)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Aly antibody [R07-5L3] (STJA0037102)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Confocal immunofluorescence analysis of U2OS cells using THOC4/THOC4/ALYREF Polyclonal Antibody (STJ27806) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALYREF antibody (1-100) (STJ27806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of LOVO cells using ALY Polyclonal Antibody

Anti-ALYREF antibody (121-170 aa) (STJ91573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of THOC4/ALYREF in paraffin-embedded human esophagus tissue using THOC4/ALYREF Rabbit monoclonal antibody (STJ11102036) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-ALYREF antibody (158-257) [S6MR] (STJ11102036)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of mouse kidney using AMACR Rabbit polyclonal antibody (STJ22599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AMACR antibody (1-138) (STJ22599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot with Anti-AMACR Antibody (STJ500070) of HEK293 cell at 1:500 dilution in buffer.

Anti-AMACR antibody (260-310) (STJ500070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£400.50

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