• Immunohistochemistry analysis of paraffin-embedded Human placenta using Placental alkaline phosphatase (PLAP) antibody (STJ11102038) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse testis, using Placental alkaline phosphatase (PLAP) Rabbit monoclonal antibody (STJ11102038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of PC-3 cells, using Placental alkaline phosphatase (PLAP) Rabbit monoclonal antibody (STJ11102038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALPP antibody (100-200) [S8MR] (STJ11102038)

SKU:
STJ11102038

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Placental alkaline phosphatase (PLAP) (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALPP
Gene ID: 250
Uniprot ID: PPB1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Placental alkaline phosphatase (PLAP) (P05187).
Immunogen Sequence: ALSKTYNVDKHVPDSGATAT AYLCGVKGNFQTIGLSAAAR FNQCNTTRGNEVISVMNRAK KAGKSVGVVTTTRVQHASPA GTYAHTVNRNWYSDADVPAS A
Tissue Specificity Detected in placenta (at protein level).
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Protein Name Alkaline Phosphatase - Placental Type
Alkaline Phosphatase Regan Isozyme
Placental Alkaline Phosphatase 1
Plap-1
Database Links Reactome: R-HSA-6811438
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Alkaline Phosphatase - Placental Type antibody
Anti-Alkaline Phosphatase Regan Isozyme antibody
Anti-Placental Alkaline Phosphatase 1 antibody
Anti-Plap-1 antibody
Anti-ALPP antibody
Anti-PLAP antibody

Information sourced from Uniprot.org

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