• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g Placental alkaline phosphatase (PLAP) antibody (STJ11104336). Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase (PLAP) antibody (STJ11104336) at a dilution of 1:1000.
  • Western blot analysis of lysates from HeLa cells, using Placental alkaline phosphatase (PLAP) Rabbit polyclonal antibody (STJ11104336) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ALPP antibody (260-460) (STJ11104336)

SKU:
STJ11104336

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alkaline Phosphatase-Placental Type (260-460) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALPP
Gene ID: 250
Uniprot ID: PPB1_HUMAN
Immunogen Region: 260-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human Placental alkaline phosphatase (PLAP) (NP_001623.3).
Immunogen Sequence: LAKRQGARYVWNRTELMQAS LDPSVTHLMGLFEPGDMKYE IHRDSTLDPSLMEMTEAALR LLSRNPRGFFLFVEGGRIDH GHHESRAYRALTETIMFDDA IERAGQLTSEEDTLSLVTAD HSHVFSFGGYPLRGSSIFGL APGKARDRKAYTVLLYGNGP GYVLKDGARPDVTESESGSP EYRQQSAVPLDEETHAGEDV A
Tissue Specificity Detected in placenta (at protein level).
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Protein Name Alkaline Phosphatase - Placental Type
Alkaline Phosphatase Regan Isozyme
Placental Alkaline Phosphatase 1
Plap-1
Database Links Reactome: R-HSA-6811438
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Alkaline Phosphatase - Placental Type antibody
Anti-Alkaline Phosphatase Regan Isozyme antibody
Anti-Placental Alkaline Phosphatase 1 antibody
Anti-Plap-1 antibody
Anti-ALPP antibody
Anti-PLAP antibody

Information sourced from Uniprot.org

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