Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts of K-562 cells, using Chk1 antibody (STJ11104686) at1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CHEK1 antibody (250-350) [S4686RM] (STJ11104686)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of MCF7 cells using Chk1 Polyclonal Antibody

Anti-CHEK1 antibody (286-335 aa) (STJ92267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ71518 (2µg/ml) staining of Rat Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHEK1 antibody (Internal) (STJ71518)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

$421.88

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Western blot analysis using Chk1 monoclonal antibody against A431 (1) , HeLa (2) , NIH/3T3 (3) and K562 (4) cell lysate.

Anti-CHEK1 antibody [2G1D5] (STJ97955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$194.40 - $469.80

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Western blot analysis of HeLa, using Chk2 antibody (STJ11101688) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHEK2 antibody (1-100) [S8MR] (STJ11101688)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Western blot analysis of various lysates, using Chek2 Rabbit polyclonal antibody (STJ11105188) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Chek2 antibody (1-215 aa) (STJ11105188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells using 1 Mu g Chk2 antibody (STJ114888). Western blot was performed from the immunoprecipitate using Chk2 antibody (STJ114888) at a dilution of 1:1000.

Anti-CHEK2 antibody (1-220) (STJ114888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of Chk2 Polyclonal Antibody, using Hela, HCT116, SW480, HEK293 cell, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-CHEK2 antibody (35-84 aa) (STJ92273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunocytochemistry analysis of Chk2 (green) in HCT116 using Chk2 antibody, and DAPI (blue).

Anti-CHEK2 antibody (43-167 aa) [R06-1B4] (STJA0011138)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$332.78

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Western blot analysis using Chk2 monoclonal antibody against cell lysate from various cell types.

Anti-CHEK2 antibody (481-531 aa) [1C12B8] (STJ97956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of various lysates using CHERP Rabbit pAb (STJ111795) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CHERP antibody (650-750) (STJ111795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot with Anti-CHERP Antibody (STJ500534) at 1 pg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 ug.

Anti-CHERP antibody (846-913) (STJ500534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$577.13

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Anti-CHERP antibody (846-913) {Biotin} (STJ500535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Anti-CHERP antibody (846-913) {FITC} (STJ500536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Western Blot analysis of hela and mouse brain cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-CHERP antibody (STJ190622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using CHFR Rabbit polyclonal antibody (STJ112476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CHFR antibody (1-290) (STJ112476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-CHFR antibody (Internal) (STJ92263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using CHGA Rabbit polyclonal antibody (STJ116155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHGA antibody (230-380) (STJ116155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various cells using Chr-A Polyclonal Antibody

Anti-CHGA antibody (311-360 aa) (STJ92281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using CHGB Rabbit polyclonal antibody (STJ23122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHGB antibody (1-300) (STJ23122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CHGB antibody (STJ193172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of Raw264. 7 cells using YKL-40/ YKL-40/CHI3L1 antibody (STJ11101169) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHI3L1 antibody (22-383) (STJ11101169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of THP-1 cells using YKL-40/CHI3L1 Rabbit polyclonal antibody (STJ116193) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHI3L1 antibody (22-383) (STJ116193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of THP-1 cells, using YKL-40/CHI3L1 antibody (STJ23123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHI3L1 antibody (22-383) (STJ23123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CHI3L1 antibody (STJ193191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of Jurkat, using CHI3L2 rabbit polyclonal antibody (STJ29968) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CHI3L2 antibody (27-390) (STJ29968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CHIA antibody (STJ116205) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CHIA antibody (1-368) (STJ116205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using CHIA antibody (STJ28641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CHIA antibody (1-368) (STJ28641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-CHIA antibody (STJA0007668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-CHIC1 antibody (51-101 aa) (STJ197627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from mouse testis, using CHIC2 Rabbit pAb (STJ110278) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-CHIC2 antibody (1-165) (STJ110278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CHIC2 antibody (111-161 aa) (STJ194199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of Chicken IgY Fab, using Chicken IgY Fab antibody (STJ11105911) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Chicken IgY-Fab antibody [S5911RM] (STJ11105911)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

$61.43 - $93.83

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Western blot analysis of various lysates using Rabbit Anti-Chicken IgY (H+L) (STJ11105912) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 5 ng- 10 ng per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Chicken IgY-H & L antibody [S5912RM] (STJ11105912)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

$61.43 - $93.83

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Western blot analysis of recombinant Chicken IL12B Protein using Chicken IL12B Rabbit monoclonal antibody (STJ11105564) at 1:1000 dilution. NA NA Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. NA NA Lysates/proteins: 10ng/5ng/1ng/0. 1ng per lane. NA NA Blocking buffer: 3% nonfat dry milk in TBST. NA NA Detection: ECL Basic Kit. NA NA Exposure time: 15s.

Anti-Chicken IL12B antibody (21-315) (STJ11105564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Chicken

$174.83 - $867.38

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Western blot analysis of lysates from mouse pancreas, using CHID1 Rabbit polyclonal antibody (STJ118582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHID1 antibody (1-393) (STJ118582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot analysis of 3T3 cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-CHID1 antibody (81-130 aa) (STJ98662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Chips antibody [JCC1] (STJ16100791)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/ELISA/IHC-P/WB

$978.08

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Immunohistochemistry analysis of CHIT1 in paraffin-embedded human normal stomach using CHIT1 Rabbit polyclonal antibody (STJ23126) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CHIT1 antibody (217-466) (STJ23126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis using Chitotriosidase monoclonal antibody against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466) -hIgGFc transfected CHO-K1 cell lysate (2).

Anti-CHIT1 antibody (22-137 aa) [1D9G2/1D9G4/2B4G3] (STJ97954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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STJ72702 (1µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHIT1 antibody (409-423) (STJ72702)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Western blot analysis of various lysates using CHKA Rabbit polyclonal antibody (STJ117234) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHKA antibody (1-170) (STJ117234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-CHKA antibody (STJA0006871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-CHKB antibody (1-100 aa) (STJ11105928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various cells using ChoKB Polyclonal Antibody

Anti-CHKB antibody (301-350 aa) (STJ92275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-CHL1 antibody (140-220 aa) (STJ191053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using CHL1 Rabbit pAb (STJ111786) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CHL1 antibody (690-950) (STJ111786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 293T cells, using (STJ11105752) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CHM antibody (1-100) [S5752RM] (STJ11105752)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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