Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of A549 cells, using ECRG4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ECRG4 antibody (41-90 aa) (STJ92822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of HepG2 cells, using ECSIT antibody (STJ110114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ECSIT antibody (50-296) (STJ110114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ70551 (1µg/ml) staining of Human lung (A) and Human Umbilical Cord (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour.

Anti-ECSM2 antibody (C-Term) (STJ70551)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human

£312.50

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Anti-ECT2 antibody (N-Term) (STJ70379)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of mouse-lung mouse-heart mouse-liver lysis using EDA antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-EDA antibody (120-170 aa) (STJ97656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of HepG2 cells, using EDA antibody (STJ23467) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EDA antibody (62-182) (STJ23467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using EDEM2 Rabbit polyclonal antibody (STJ11102871) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EDEM2 antibody (399-578) (STJ11102871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-EDEM2 antibody (C-Term) (STJ92823)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ73639 (0. 5µg/ml) staining of HEK293 (A) , HeLa (B) and (1ug/ml) NIH3T3 (C) Nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-EDF1/MBF1 antibody (N-Term) (STJ73639)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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Immunofluorescence analysis of U2OS cells using EDIL3 antibody (STJ118217) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EDIL3 antibody (350-450) (STJ118217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of mouse lung cells using ET-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-EDN1 antibody (131-180 aa) (STJ96512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit polyclonal antibody (STJ119977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-EDN1 antibody (18-212) (STJ119977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Endothelin 1 Rabbit polyclonal antibody (STJ23471) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EDN1 antibody (18-212) (STJ23471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using ETAR Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-EDNRA antibody (378-427 aa) (STJ93004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Prostate Tissue using Endothelin B ReceptorRabbit pAb diluted at 1:200.

Anti-EDNRB antibody (270-350 aa) (STJ197396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of Hela cells using ETBR Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-EDNRB antibody (31-80 aa) (STJ98617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Endogenous EEA1 detected with antibody at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EEA1 antibody (1230aa C-Term) (STJ140033)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Anti-EEA1 antibody at 1:500 dilution; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EEA1 antibody (1230aa C-Term) (STJ140034)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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STJ73333 (0. 3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-EEA1 antibody (821-835) (STJ73333)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human

£312.50

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Immunofluorescence analysis of U-2 OS cells using EED Rabbit polyclonal antibody (STJ114646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EED antibody (1-100) (STJ114646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using EED rabbit polyclonal antibody (STJ27324) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EED antibody (1-400) (STJ27324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit polyclonal antibody (STJ23475) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EEF1A1 antibody (123-462) (STJ23475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of C6 cells using eEF1A1 Rabbit monoclonal antibody (STJ11101355, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-EEF1A1 antibody (250-350) [S5MR] (STJ11101355)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using eEF1A1 antibody (STJ119870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EEF1A1 antibody (301-400) (STJ119870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of Elongation Factor 1A1 (green) in Hela using Elongation Factor 1A1 antibody, and DAPI (blue).

Anti-EEF1A1 antibody (325-338 aa) [R04-3H4] (STJA0012354)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£264.50

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Immunohistochemistry analysis of EEF1A2 in paraffin-embedded rat brain tissue using EEF1A2 rabbit polyclonal antibody (STJ11100935) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-EEF1A2 antibody (184-463) (STJ11100935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using EEF1B2 antibody (STJ28663) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EEF1B2 antibody (1-225) (STJ28663)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunoprecipitation of EEF1D in 300 µg extracts from Jurkat cells using 3 µg EEF1D Rabbit polyclonal antibody (STJ23477). Western blot analysis was performed using EEF1D Rabbit polyclonal antibody (STJ23477) at 1:500 dilution.

Anti-EEF1D antibody (1-160) (STJ23477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using EEF1E1 Rabbit polyclonal antibody (STJ117552) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EEF1E1 antibody (1-100) (STJ117552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using eEF1G Rabbit mAb (STJ11102367) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-EEF1G antibody (1-123) [S7MR] (STJ11102367)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using eEF1G Rabbit pAb (STJ113571) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-EEF1G antibody (168-437) (STJ113571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using EF-2 Polyclonal Antibody diluted at 1：2000

Anti-EEF2 antibody (31-80 aa) (STJ92841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using eEF2 Rabbit mAb (STJ11102532) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EEF2 antibody (759-858) [S2MR] (STJ11102532)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot detection of eEF2 in HL-60, Jurkat, SHSY-5Y, U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.

Anti-EEF2 antibody [4B3-G7-H5] (STJ99117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using eEF2K Polyclonal Antibody diluted at 1：500

Anti-EEF2K antibody (331-380 aa) (STJ92839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A-549 cells using EEF2K antibody (STJ27357). Blue: DAPI for nuclear staining.

Anti-EEF2K antibody (406-725) (STJ27357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of rat cells using Fibulin-3 Polyclonal Antibody

Anti-EFEMP1 antibody (111-160 aa) (STJ93073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using EFEMP2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EFEMP2 antibody (91-140 aa) (STJ93074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, EFHD1 monoclonal antibody (3G2) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EFHD1 antibody [3G2] (STJ96987)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Ephrin-A1 Polyclonal Antibody diluted at 1：2000

Anti-EFNA1 antibody (66-115 aa) (STJ92954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Ephrin-A3 Polyclonal Antibody

Anti-EFNA3 antibody (161-210 aa) (STJ92956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Ephrin-A5 Polyclonal Antibody

Anti-EFNA5 antibody (31-80 aa) (STJ92958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of SKOV3 cells using Ephrin-B3 Polyclonal Antibody

Anti-EFNB3 antibody (221-270 aa) (STJ92961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-EFTUD2 antibody (1-205 aa) [SRM] (STJ11107172)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 300 Mu g extracts of NTH-3T3 cells using 3 Mu g EFTUD2 Rabbit polyclonal antibody (STJ29120). Western blot was performed from the immunoprecipitate using EFTUD2 Rabbit polyclonal antibody (STJ29120) at a dilition of 1:2000.

Anti-EFTUD2 antibody (1-205) (STJ29120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Snrp116 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-EFTUD2 antibody (321-370 aa) (STJ95722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-EGF antibody (1000-1060 aa) (STJ98714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of human skin using EGF Rabbit polyclonal antibody (STJ115574) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EGF antibody (901-916) (STJ115574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options