Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemistry analysis of IBA-1. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Iba1 antibody [SM4917] (STJA0004917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IF-P

Reactivity :

Human/Mouse/Rat/Monkey

£170.50 - £233.50

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Anti-ICAM-1/CD54 antibody [SRM] (STJ11106871)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using ICAM-1/CD54 Rabbit mAb (STJ11106969) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-ICAM-1/CD54 antibody [SRM] (STJ11106969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-ICAM-1/CD54 antibody [SRM] (STJ11107208)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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HUVEC whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD54 (ABT-CD54) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HUVECPredicted band size: 58kDaObserved band size: 100kDa

Anti-ICAM1 antibody (100-200 aa) [ABT-CD54] (STJ196917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of various lysates, using ICAM-1/CD54 antibody (STJ27566) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-ICAM1 antibody (28-480) (STJ27566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded Rat lung tissue using Icam1 Rabbit polyclonal antibody (STJ11105185) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Icam1 antibody (28-493 aa) (STJ11105185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ73132 (0. 3µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ICAM1 antibody (313-327) (STJ73132)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of various cells using ICAM-1 Polyclonal Antibody diluted at 1：500

Anti-ICAM1 antibody (479-528 aa) (STJ93634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ72888 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ICAM1 antibody (Internal) (STJ72888)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human

£312.50

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Western blot analysis using ICAM-1 monoclonal antibody against HUVEC cell lysate.

Anti-ICAM1 antibody (N-Term) (STJ98507)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

£144.00 - £348.00

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Anti-ICAM1 antibody [PT0133R] (STJA0039737)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

£0.00

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Immunohistochemistry analysis of paraffin embedded human tonsil using ICAM1 at dilution of 1:200

Anti-ICAM1 antibody [SM5148] (STJA0005148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of NIH-3T3 cells using CD50 Polyclonal Antibody

Anti-ICAM3 antibody (484-533 aa) (STJ92132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-colon, antibody was diluted at 1:100

Anti-ICAM4 antibody (141-190 aa) (STJ99004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit polyclonal antibody (STJ24118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ICOS antibody (21-140) (STJ24118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 lysis using ICOS antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-ICOS antibody (31-80 aa) (STJ97666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-ICOSLG antibody (201-250 aa) (STJ99001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-ID2B antibody (1-24 aa) (STJ196211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using ID3 antibody (STJ27328).

Anti-ID3 antibody (1-119) (STJ27328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ID4 antibody (1-50 aa) (STJ93637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) antibody (STJ24120). Blue: DAPI for nuclear staining.

Anti-IDE antibody (1-250) (STJ24120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IDE antibody [3H4] (STJ96985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Hamster

£48.00 - £252.00

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Anti-IDE antibody [PT1295R] (STJA0040300)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IDH1 antibody (STJ115211). Western blot was performed from the immunoprecipitate using IDH1 antibody (STJ115211) at a dilution of 1:1000.

Anti-IDH1 antibody (1-414) (STJ115211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of PC-12 cells using IDH1 Rabbit polyclonal antibody (STJ24121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDH1 antibody (100-414) (STJ24121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

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Western blot analysis of 293, HeLa cells using IDH1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH1 antibody (N-Term) (STJ96781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Jurkat, using IDH2 antibody (STJ29270) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IDH2 antibody (193-452) (STJ29270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using IDH3A Rabbit polyclonal antibody (STJ116856) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IDH3A antibody (28-366) (STJ116856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of MCF-7, HT29, mouse brain, mouse heart, mouse colon, mouse kidney, mouse skeletal muscle cells using IDH3A Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH3A antibody (Internal) (STJ96683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using IDH3B Rabbit polyclonal antibody (STJ115693) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IDH3B antibody (35-170) (STJ115693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-IDO1 antibody (1-403 aa) [SRM] (STJ11106193)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of SKOV3 cells, using IDO1 rabbit polyclonal antibody antibody (STJ24203) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IDO1 antibody (1-403) (STJ24203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using IDS Rabbit polyclonal antibody (STJ24123) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDS antibody (34-290) (STJ24123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of IER5 in paraffin-embedded Rat pancreas using IER5 Rabbit polyclonal antibody (STJ11100411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IER5 antibody (200-300) (STJ11100411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-IFI27 antibody (2-13) (STJ501398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-IFI27 antibody (2-13) {Biotin} (STJ501400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-IFI27 antibody (2-13) {FITC} (STJ501401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Western blot analysis of various lysates using IFI44 Rabbit pAb (STJ110487) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-IFI44 antibody (165-181) (STJ110487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of THP-1 treated with LPS (32h) and THP-1 cells using IFIH1 Rabbit polyclonal antibody (STJ115604) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IFIH1 antibody (1-1025) (STJ115604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using MDA5 Polyclonal Antibody

Anti-IFIH1 antibody (976-1025 aa) (STJ94052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-IFIT1 antibody (41-90 aa) (STJ93640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of MCF7, PC12 cells using CD225 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IFITM1 antibody (1-50 aa) (STJ96843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] IFITM3 Rabbit polyclonal antibody (STJ115037) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IFITM3 antibody (1-133) (STJ115037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using IFITM3 Polyclonal Antibody diluted at 1：2000

Anti-IFITM3 antibody (1-50 aa) (STJ93642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-IFITM3 antibody [PT0253R] (STJA0039716)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-IFN Gamma Receptor/CD119 antibody [A6C6] (STJ16101997)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£636.50

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Immunofluorescence analysis of paraffin-embedded mouse small intestine using IFN-beta Rabbit polyclonal antibody (STJ11105175) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IFN-beta antibody (1-182) (STJ11105175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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