• Western blot analysis of extracts of various cell lines, using GABPA antibody (STJ110717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of HeLa cells using GABPA Polyclonal Antibody (STJ110717) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human uterine cancer using GABPA antibody (STJ110717) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GABPA antibody (STJ110717) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using GABPA antibody (STJ110717) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human uterine cancer using GABPA antibody (STJ110717) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using GABPA Polyclonal Antibody (STJ110717) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GABPA antibody (STJ110717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GABPA antibody (1-260) (STJ110717)

SKU:
STJ110717

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GABPA (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABPA
Gene ID: 2551
Uniprot ID: GABPA_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GABPA (NP_002031.2).
Immunogen Sequence: MTKREAEELIEIEIDGTEKA ECTEESIVEQTYAPAECVSQ AIDINEPIGNLKKLLEPRLQ CSLDAHEICLQDIQLDPERS LFDQGVKTDGTVQLSVQVIS YQGIEPKLNILEIVKPADTV EVVIDPDAHHAESEAHLVEE AQVITLDGTKHITTISDETS EQVTRWAAALEGYRKEQERL GIPYDPIQWSTDQVLHWVVW VMKEFSMTDIDLTTLNISGR ELCSLNQEDFFQRVPRGEI
Function Transcription factor capable of interacting with purine rich repeats (GA repeats). Positively regulates transcription of transcriptional repressor RHIT/ZNF205. (Microbial infection) Necessary for the expression of the Adenovirus E4 gene.
Protein Name Ga-Binding Protein Alpha Chain
Gabp Subunit Alpha
Nuclear Respiratory Factor 2 Subunit Alpha
Transcription Factor E4tf1-60
Database Links Reactome: R-HSA-2151201
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ga-Binding Protein Alpha Chain antibody
Anti-Gabp Subunit Alpha antibody
Anti-Nuclear Respiratory Factor 2 Subunit Alpha antibody
Anti-Transcription Factor E4tf1-60 antibody
Anti-GABPA antibody
Anti-E4TF1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance