• STJ71191 (0. 3µg/ml) staining of HeLa cell lysate (A) + peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71191 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear/nuclear speckle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71191 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71191 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-GABPB2 antibody (Internal) (STJ71191)

SKU:
STJ71191-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GABPB2 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GABPB1
Gene ID: 2553
Uniprot ID: GABP1_HUMAN
Immunogen Region: Internal
Accession Number: NP_005245.2; NP_002032.2; NP_001307844.1
Immunogen Sequence: SSENSSKATDETG
Function Transcription factor capable of interacting with purine rich repeats (GA repeats). Acts as a a master regulator of nuclear-encoded mitochondrial genes. (Microbial infection) Necessary for the expression of the Adenovirus E4 gene.
Protein Name Ga-Binding Protein Subunit Beta-1
Gabp Subunit Beta-1
Gabpb-1
Gabp Subunit Beta-2
Gabpb-2
Nuclear Respiratory Factor 2
Transcription Factor E4tf1-47
Transcription Factor E4tf1-53
Database Links Reactome: R-HSA-2151201
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ga-Binding Protein Subunit Beta-1 antibody
Anti-Gabp Subunit Beta-1 antibody
Anti-Gabpb-1 antibody
Anti-Gabp Subunit Beta-2 antibody
Anti-Gabpb-2 antibody
Anti-Nuclear Respiratory Factor 2 antibody
Anti-Transcription Factor E4tf1-47 antibody
Anti-Transcription Factor E4tf1-53 antibody
Anti-GABPB1 antibody
Anti-E4TF1B antibody
Anti-GABPB antibody
Anti-GABPB2 antibody

Information sourced from Uniprot.org

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