ELISA Antibodies | St John's Laboratory

Immunofluorescence analysis of U-937 cells using CD209 Rabbit polyclonal antibody (STJ111093) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD209 antibody (265-404) (STJ111093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using CD22 Rabbit polyclonal antibody (STJ11105561) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : Jurkat. Exposuretime: 60s.

Anti-CD22 antibody (1-100) (STJ11105561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various lysates using CD22 Rabbit mAb (STJ11107296) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Negative control (NC) : Jurkat<br>Exposure time: 60s.

Anti-CD22 antibody (20-687 aa) [SRM] (STJ11107296)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunofluorescence analysis of Raji cells using CD22 Rabbit polyclonal antibody (STJ28354) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD22 antibody (708-847) (STJ28354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of 293 cells using CD22 Polyclonal Antibody

Anti-CD22 antibody (776-825 aa) (STJ92105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-CD22 antibody [SRM] (STJ11107213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Western blot analysis of extracts of mouse lung, using CD226 antibody (STJ112320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD226 antibody (19-254) (STJ112320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot analysis of various lysates using CD226 Rabbit pAb (STJ115360) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CD226 antibody (19-336) (STJ115360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of COS7 cells using DNAM-1 Polyclonal Antibody diluted at 1：500

Anti-CD226 antibody (287-336 aa) (STJ92746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Western blot analysis of HeLa cells using DNAM-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD226 antibody (71-120 aa) (STJ96767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates, using CD226/DNAM-1 Rabbit monoclonal antibody (STJ11105046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD226 antibody [S5046RM] (STJ11105046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Anti-CD227 antibody [C595] (STJ16100530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC/IHC-F/ELISA/IF

Reactivity :

Human

$1,266.98

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Anti-CD239 antibody [BRIC221] (STJ16100542)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/IF/FC/IHC-F

Reactivity :

Human/Pig

$1,001.03

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Immunohistochemistry analysis of paraffin-embedded human oophoroma using CD239/BCAM Rabbit monoclonal antibody (STJ11103094) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD239/BCAM antibody (529-628) [S4MR] (STJ11103094)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Anti-CD24 antibody (1-80 aa) [SRM] (STJ11107235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Confocal imaging of MCF7 cells using CD24 Rabbit mAb (STJ11107374, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Î±-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-CD24 antibody (1-80 aa) [SRM] (STJ11107374)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD24 Rabbit polyclonal antibody (STJ22979) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD24 antibody (1-80) (STJ22979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysate from NIH/3T3 cells, using CD24 antibody.

Anti-CD24 antibody (14-63 aa) (STJ92106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from Mouse liver using mouse CD24 Rabbit polyclonal antibody (STJ11105518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD24 antibody (27-53) (STJ11105518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-CD24 antibody (30-60 aa) (STJ98990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of KB cells using CD244 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD244 antibody (101-150 aa) (STJ97310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from U-937 cells using CD244 Rabbit mAb (STJ11107322) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 180s.

Anti-CD244 antibody (22-221 aa) [SRM] (STJ11107322)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of various lysates, using CD244 antibody (STJ22980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD244 antibody (22-224) (STJ22980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from Jurkat cells, treated with UV 15', using CD3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CD247 antibody (111-160 aa) (STJ92112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunofluorescence analysis of A549 cells using CD3H antibody (STJ22982). Blue: DAPI for nuclear staining.

Anti-CD247 antibody (50-164) (STJ22982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Flow cytometry:1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3H Rabbit monoclonal antibody (STJ11101284, 10 Mu g/mL, orange line) or Rabbit IgG isotype control ( 10 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-CD247 antibody (65-164) [S4MR] (STJ11101284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis using CD247 monoclonal antibody against CD247-hIgGFc transfected HEK293 cell lysate.

Anti-CD247 antibody [4B10] (STJ97916)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Flow cytometric analysis CD3 expressed in human peripheral blood cells using CD3 monoclonal antibody.

Anti-CD247 antibody [4D10A6] (STJ97918)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Immunofluorescence analysis of peripheral blood T cells using CD3 monoclonal antibody.

Anti-CD247 antibody [4D10A6] {FITC} (STJ97919)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of extracts of various cell lines, using CD248 antibody (STJ114576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from HeLa cells, using CD248 Rabbit polyclonal antibody (STJ114579) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of HepG2 cells using CD248 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD248 antibody (341-390 aa) (STJ96632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-CD25/IL2RA antibody (117-129) (STJ73525)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human

$261.23

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Anti-CD25/IL2RA antibody [143-13] (STJ16102559)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FUNC/WB/IF/IHC

Reactivity :

Human

$388.13 - $1,788.08

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Anti-CD25/IL2RA antibody [EBS-CD-017] (STJ16101100)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF/IHC

Reactivity :

Human

$388.13 - $1,788.08

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Anti-CD26 antibody [5+00E+08] (STJ16100784)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IP/WB

Reactivity :

Rat

$894.38

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Confocal imaging of human tonsil using CD27 Rabbit monoclonal antibody (STJ11101350, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-CD27 antibody (1-100) [S0MR] (STJ11101350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$174.83 - $775.58

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD27 antibody (1-80 aa) (STJ191672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD27 (PT1818) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: K562Predicted band size: 29kDaObserved band size: 40kDa

Anti-CD27 antibody (160-260) [PT1818] (STJA0006232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$64.80 - $340.20

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STJ73070 (0. 3µg/ml) staining of Human Spleen (A) , Tonsil (B) and Thymus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD27 antibody (161-175) (STJ73070)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Western blot analysis of lysates from Mouse lung, using CD27 Rabbit polyclonal antibody (STJ118100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD27 antibody (20-191) (STJ118100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (20-191) (STJ22984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of CD271 in paraffin-embedded Human tonsil tissue using CD271 Rabbit pAb (STJ11107099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CD271 antibody (32-254 aa) (STJ11107099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

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Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD271/NGFR Rabbit monoclonal antibody (STJ11105254, 2 Mu g/mL, right).

Anti-CD271 antibody (32-254) [S5254RM] (STJ11105254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse/Rat

$174.83 - $775.58

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Anti-CD274 antibody (181-230 aa) (STJ98996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit monoclonal antibody (STJ11105258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (19-235) [S5258RM] (STJ11105258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$174.83 - $775.58

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ113753) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (19-238) (STJ113753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using CD274 antibody (STJ11103333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD274 antibody (19-239) (STJ11103333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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