Western blot analysis of various lysates using CCL27 Rabbit pAb (STJ111906) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CCL27 antibody (25-112) (STJ111906)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111906
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General Information

Short DescriptionRabbit polyclonal CCL27 (25-112) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCCL27
Gene ID10850
Uniprot IDCCL27_HUMAN
Immunogen Region25-112
Immunogen SequenceFLLPPSTACCTQLYRKPLSD KLLRKVIQVELQEADGDCHL QAFVLHLAQRSICIHPQNPS LSQWFEHQERKLHGTLPKLN FGMLRKMG
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 25-112 of human CCL27 (NP_006655.1).

Additional Info

Tissue Specificity Testis, thymus, placenta, ovary and skin.
Function Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Protein Name C-C Motif Chemokine 27
Cc Chemokine Ilc
Cutaneous T-Cell-Attracting Chemokine
Ctack
Eskine
Il-11 R-Alpha-Locus Chemokine
Skinkine
Small-Inducible Cytokine A27
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative Antibody Names Anti-C-C Motif Chemokine 27 antibody
Anti-Cc Chemokine Ilc antibody
Anti-Cutaneous T-Cell-Attracting Chemokine antibody
Anti-Ctack antibody
Anti-Eskine antibody
Anti-Il-11 R-Alpha-Locus Chemokine antibody
Anti-Skinkine antibody
Anti-Small-Inducible Cytokine A27 antibody
Anti-CCL27 antibody
Anti-ILC antibody
Anti-SCYA27 antibody

Information sourced from Uniprot.org

Citations

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