• Immunohistochemistry analysis of CCL21 in paraffin-embedded human stomach using CCL21 Rabbit polyclonal antibody (STJ22930) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CCL21 in paraffin-embedded human liver using CCL21 Rabbit polyclonal antibody (STJ22930) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of recombinant Human CCL21 Protein using CCL21 Rabbit polyclonal antibody (STJ22930) at 1:1000 dilution. NA NA Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. NA NA Lysates/proteins: 100 ng/10 ng/1 ng per lane. NA NA Blocking buffer: 3% nonfat dry milk in TBST. NA NA Detection: ECL Basic Kit. NA NA Exposure time: 90s.

Anti-CCL21 antibody (24-134) (STJ22930)

SKU:
STJ22930

£112.50 - £522.50
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Host: Rabbit
Applications: IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCL21 (24-134) is suitable for use in Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCL21
Gene ID: 6366
Uniprot ID: CCL21_HUMAN
Immunogen Region: 24-134
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-134 of human CCL21 (NP_002980.1).
Immunogen Sequence: SDGGAQDCCLKYSQRKIPAK VVRSYRKQEPSLGCSIPAIL FLPRKRSQAELCADPKELWV QQLMQHLDKTPSPQKPAQGC RKDRGASKTGKKGKGSKGCK RTERSQTPKGP
Tissue Specificity Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.
Function Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Protein Name C-C Motif Chemokine 21
6ckine
Beta-Chemokine Exodus-2
Secondary Lymphoid-Tissue Chemokine
Slc
Small-Inducible Cytokine A21
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative Antibody Names Anti-C-C Motif Chemokine 21 antibody
Anti-6ckine antibody
Anti-Beta-Chemokine Exodus-2 antibody
Anti-Secondary Lymphoid-Tissue Chemokine antibody
Anti-Slc antibody
Anti-Small-Inducible Cytokine A21 antibody
Anti-CCL21 antibody
Anti-SCYA21 antibody
Anti-UNQ784 antibody
Anti-PRO1600 antibody

Information sourced from Uniprot.org

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