Human Macrophage migration inhibitory factor/MIF protein (Recombinant) (No tag) (STJP003061)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant human Macrophage migration inhibitory factor/MIF Protein is produced by E.coli expression system. The target protein is expressed with sequence (Met1-Ala115) of human Macrophage migration inhibitory factor/MIF (Accession #NP_002406.1) fus
General Information
| Short Description | Recombinant-Human Macrophage migration inhibitory factor/MIF-No tag protein was developed in e.coli using the region Met1-Ala115. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM PB, 150mM NaCl, pH 7.4/20mM PB, 150mM NaCl, pH 7.4, 50% Glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MIF |
| Gene ID | 4282 |
| Uniprot ID | MIF_HUMAN |
| Immunogen | Recombinant human Macrophage migration inhibitory factor/MIF Protein is produced by E.coli expression system. The target protein is expressed with sequence (Met1-Ala115) of human Macrophage migration inhibitory factor/MIF (Accession #NP_002406.1) fus |
| Immunogen Region | Met1-Ala115 |
| Immunogen Sequence | PGPPAGSPRVSSDPRADLDS AVLLTRSLLADTRQLAAQMR DKFPADGDHSLDSLPTLAMS AGTLGSLQLPGVLTRLRVDL MSYLRHVQWLRRAGGPSLKT LEPELGALQARLERLLRRLQ LLMSRLALPQAAPDQPVIPL GPPASAWGSIRAAHAILGGL HLTLDWAVRGLLLLKTRL |
Additional Info
| Function | Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
| Protein Name | Macrophage Migration Inhibitory FactorMifGlycosylation-Inhibiting FactorGifL-Dopachrome IsomeraseL-Dopachrome TautomerasePhenylpyruvate Tautomerase |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-6798695Reactome: R-HSA-8950505 |
| Cellular Localisation | SecretedCytoplasmDoes Not Have A Cleavable Signal Sequence And Is Secreted Via A SpecializedNon-Classical PathwaySecreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps)Or By MTuberculosis Antigens |
| Alternative Protein Names | Macrophage Migration Inhibitory Factor proteinMif proteinGlycosylation-Inhibiting Factor proteinGif proteinL-Dopachrome Isomerase proteinL-Dopachrome Tautomerase proteinPhenylpyruvate Tautomerase proteinMIF proteinGLIF proteinMMIF protein |
Information sourced from Uniprot.org