DyLight Conjugated Secondary Antibodies
Bright, multi-colour fluorescent secondaries for western blot, flow cytometry, immunofluorescence and ELISA
DyLight dyes are amine-reactive fluorophores conjugated directly onto the secondary antibody, so each dye molecule is excited at a set wavelength and emits at a longer one, giving a strong signal that a fluorescence microscope, flow cytometer or infrared imager can read out. Our DyLight range spans the visible spectrum from 350 nm through to the 800 nm near-infrared channel, across goat, chicken and rabbit host secondaries reactive against mouse, rabbit, rat, goat and chicken IgG or IgY, so a panel built entirely from DyLight conjugates can assign a separate host reactivity and colour channel to each primary antibody in the same experiment.
Browse DyLight Secondary AntibodiesTwo Channels for Building a Multiplex Panel
DyLight 405 sits at the blue end of the range, matched to a violet 405 nm laser line, while DyLight 633 sits at the far-red end, matched to a 633 nm laser line. Run together with a green- or yellow-channel conjugate in between, they anchor a multi-colour panel where each channel is read without bleeding into the next.
Goat Anti-Mouse IgG (Full length) Antibody {DyLight®405}
DyLight 405 is excited by a violet 405 nm laser line and read in the blue channel, a wavelength range typically reserved for nuclear counterstains or low-abundance mouse-raised primaries. This goat polyclonal detects full-length mouse IgG and is validated by immunofluorescence in mouse and human samples.
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Chicken Anti-Chicken IgY (Full length) Antibody {DyLight®633}
DyLight 633 is matched to a 633 nm laser line and reads separately from DAPI, DyLight 488 and DyLight 550 channels in the same panel. This chicken polyclonal detects full-length chicken IgY and is validated by immunofluorescence, giving a far-red option when the primary antibody itself is IgY-based rather than mammalian IgG.
View product →Anti-Mouse IgG DyLight Secondary Antibodies
Goat anti-mouse is the most requested reactivity in the DyLight range, pairing with mouse monoclonal primaries across western blot, flow cytometry, immunofluorescence and ELISA. The full-length format conjugates below (DyLight 405, 488, 550 and 633) are validated by immunofluorescence in mouse and human samples. The same reactivity is also supplied as a shorter IgG-only conjugate across DyLight 350, 405, 488, 549, 594, 649, 680 and 800, for applications that call for a narrower wavelength window or near-infrared detection.
Anti-Rat IgG DyLight Secondary Antibodies
Rat-raised primaries are common in neuroscience and rodent disease models, where a rat monoclonal can offer cleaner specificity than a mouse or rabbit equivalent raised against the same target. Our goat anti-rat DyLight conjugates are validated by immunofluorescence in mouse and rat samples and are supplied in the 488 and 550 full-length format below, with the 594 and 649 wavelengths available as the shorter IgG-only conjugate.
Chicken-Host DyLight Secondary Antibodies
Chicken-raised secondaries avoid the Fc receptor and complement binding that mammalian-host secondaries can pick up in mammalian tissue, because IgY lacks the effector-binding domains found in IgG. Our chicken-host DyLight conjugates cover two reactivities: chicken anti-chicken IgY, for panels built around a chicken-raised primary, and chicken anti-goat IgG, for panels built around a goat primary. Both reactivities are validated by immunofluorescence, and the anti-goat IgG conjugate is additionally validated by western blot at DyLight 633.
Western blot validated in COS-7 and HeLa lysate.
Anti-Rabbit and Anti-Goat DyLight Secondary Antibodies
When a panel already carries a goat anti-mouse or goat anti-rat conjugate in another channel, a goat anti-rabbit or rabbit anti-goat secondary keeps each channel assigned to a single host and reactivity. Our goat anti-rabbit IgG conjugates span the full DyLight wavelength set from 350 to 800, and our rabbit anti-goat IgG conjugates span 488 to 649, both supplied as the shorter IgG-only conjugate.
Used in a 2020 Cell Death & Disease study on TGF-β1/Smad signalling in cardiac fibrosis1.
Far-red channel, IgG-only conjugate.
For panels built around a goat-raised primary.
Near-Infrared Detection with DyLight 680 and 800
DyLight 680 and 800 conjugates are read on laser- and filter-based infrared imaging systems operating in the 700 nm and 800 nm channels rather than a standard fluorescence microscope, giving a western blot detection option away from the autofluorescence seen at visible wavelengths. Goat anti-mouse and goat anti-rabbit DyLight 800 conjugates were used for infrared western blot detection of exosome markers in a Signal Transduction and Targeted Therapy study on cardiomyocyte-derived exosomes and ferroptosis sensitivity in ischemic heart failure2.
Browse Other Secondary Antibody Conjugates
Where a chromogenic or biotin-streptavidin readout suits the assay better than a directly fluorescent one, our secondary antibody range is also available in Alexa Fluor, HRP, Biotin, gold-labelled and FITC formats. Select a conjugation type below to browse that part of the range.
Photostable, pH-stable fluorescent conjugates spanning 350 to 790 nm.
Browse Alexa Fluor →Chromogenic and chemiluminescent conjugates for western blot and ELISA.
Browse HRP →Biotin conjugates for streptavidin and avidin-based detection systems.
Browse Biotinylated →Colloidal gold conjugates for electron microscopy and lateral flow formats.
Browse Gold-Labelled →Green-emitting fluorescent conjugates for IF and flow cytometry.
Browse FITC →Learning and Support
Matching a conjugate to your imaging instrument
Match each DyLight conjugate to the laser line available on your instrument: a blue laser diode for DyLight 405, a cyan 488 nm laser for DyLight 488, a green 526 nm laser for DyLight 550 and 594, and a red 633 nm laser for DyLight 649. DyLight 680 and 800 conjugates need a laser- and filter-based infrared imaging instrument operating in the 700 nm and 800 nm channels rather than a standard fluorescence microscope.
Building a multi-colour panel?
For panels combining primaries raised in more than one host species, choosing conjugates across our chicken, goat and rabbit host secondaries lets each primary keep a separate host reactivity as well as a separate colour channel. Not sure which host, reactivity or wavelength fits your panel? Talk to us and our team can help you select the right products.
Looking for a Specific Reactivity or Wavelength?
Browse the full range of DyLight conjugated secondary antibodies, or contact us if a specific host, reactivity or wavelength is not listed.
Browse DyLight Secondary AntibodiesReferences
- Gao L, Wang Ly, Liu Zq, et al. TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways. Cell Death Dis. 2020;11:44.
- Signal Transduction and Targeted Therapy. Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure (Yang lab), as cited on the product datasheets for STJS000790 and STJS000791.