Chicken Anti-Goat IgG (Full length) antibody {DyLight®405} (STJ140221)
General Information
| Short Description | DyLight®405-conjugated chicken polyclonal anti-Goat IgG Full length secondary antibody. For use in most research applications. |
| Applications | WB/IF |
| Host | Chicken |
| Reactivity | Goat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | DyLight®405 |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000IF 1:100-1:1000 |
| Formulation | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full goat IgG affinity purified from domestic goat (Capra hircus) serum using protein G. Purity >95% by SDS-PAGE. |
| Immunogen Region | Full length |
| Specificity | Using serum samples detects goat IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Chicken Anti-Goat IgG (Full length) antibody {DyLight®405} (STJ140221)
This antibody is raised in chicken against goat IgG (full length) and is designed to detect primary antibodies derived from goat. The immunogen was full goat IgG affinity purified from domestic goat (Capra hircus) serum, with purity greater than 95% by SDS-PAGE.
This antibody is conjugated to DyLight 405, a violet-excitable fluorescent dye. It has been validated for use in Western blot (WB) and immunofluorescence (IF).
The recommended dilution range is 1:2000 to 1:10000 for Western blot and 1:100 to 1:1000 for immunofluorescence. Optimal dilution should be determined empirically for each experimental system.
The product details note reduced cross-reactivity to other species, but do not specify which species were tested. Researchers working with samples containing IgG from multiple species should validate the antibody in their specific system prior to use.
For continuous use, store at 2 to 8 degrees C for one to two days. For extended storage, keep at minus 20 degrees C. Working dilution samples should be discarded if not used within 12 hours.
This antibody is supplied at 2 mg/mL in PBS with 20% glycerol, 0.05% ProClin, and 0.05% sodium azide. Researchers should account for the sodium azide content if downstream enzyme-based detection systems are being used in parallel experiments.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.