Chicken Anti-Goat IgG (Full length) antibody {DyLight®488} (STJ140222)
General Information
| Short Description | DyLight®488-conjugated chicken polyclonal anti-Goat IgG Full length secondary antibody. For use in most research applications. |
| Applications | WB/IF |
| Host | Chicken |
| Reactivity | Goat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | DyLight®488 |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000IF 1:100-1:1000 |
| Formulation | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full goat IgG affinity purified from domestic goat (Capra hircus) serum using protein G. Purity >95% by SDS-PAGE. |
| Immunogen Region | Full length |
| Specificity | Using serum samples detects goat IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Chicken Anti-Goat IgG (Full length) antibody {DyLight®488} (STJ140222)
This antibody is raised in chicken and targets goat IgG (full length). It is suited for use with primary antibodies produced in goat, and was generated using full goat IgG affinity purified from domestic goat (Capra hircus) serum.
This antibody is conjugated to DyLight 488 and has been validated for Western blot (WB) and immunofluorescence (IF).
The recommended dilution is 1:2000 to 1:10000 for Western blot and 1:100 to 1:1000 for immunofluorescence. Optimal dilution should be determined empirically for each experimental system.
The product listing states reduced cross-reactivity to other species, and the antibody is described as detecting goat IgG by Western blot using serum samples. Researchers working with samples containing IgG from closely related species should validate specificity in their own system.
For continuous use, store at 2 to 8 degrees Celsius for one to two days. For extended storage, keep at -20 degrees Celsius. Prepared working dilutions should be discarded if not used within 12 hours.
This antibody is supplied in PBS with 20% glycerol, 0.05% ProClin, and 0.05% sodium azide at a concentration of 2 mg/mL. Sodium azide can inhibit horseradish peroxidase activity, so researchers using HRP-based detection in combination assays should account for this.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.