• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using ZEB1 antibody (STJ26150) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Jurkat cells, using ZEB1 Rabbit polyclonal antibody (STJ26150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ZEB1 antibody (490-710) (STJ26150)

SKU:
STJ26150

€140.63 - €655.63
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:100-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZEB1
Gene ID: 6935
Uniprot ID: ZEB1_HUMAN
Immunogen Region: 490-710
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 490-710 of human ZEB1 (NP_110378.3).
Immunogen Sequence: QNLKKENPVATNSCKSEKLP EDLTVKSEKDKSFEGGVNDS TCLLCDDCPGDINALPELKH YDLKQPTQPPPLPAAEAEKP ESSVSSATGDGNLSPSQPPL KNLLSLLKAYYALNAQPSAE ELSKIADSVNLPLDVVKKWF EKMQAGQISVQSSEPSSPEP GKVNIPAKNNDQPQSANANE PQDSTVNLQSPLKMTNSPVL PVGSTTNGSRSSTPSPSPLN L
Tissue Specificity Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Post Translational Modifications Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization.
Function Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region.
Protein Name Zinc Finger E-Box-Binding Homeobox 1
Nil-2-A Zinc Finger Protein
Negative Regulator Of Il2
Transcription Factor 8
Tcf-8
Database Links Reactome: R-HSA-6785807
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger E-Box-Binding Homeobox 1 antibody
Anti-Nil-2-A Zinc Finger Protein antibody
Anti-Negative Regulator Of Il2 antibody
Anti-Transcription Factor 8 antibody
Anti-Tcf-8 antibody
Anti-ZEB1 antibody
Anti-AREB6 antibody
Anti-TCF8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance