Anti-ZEB1 antibody (490-710) (STJ26150)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ZEB1 (490-710) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZEB1 |
| Gene ID | 6935 |
| Uniprot ID | ZEB1_HUMAN |
| Immunogen Region | 490-710 |
| Immunogen Sequence | QNLKKENPVATNSCKSEKLP EDLTVKSEKDKSFEGGVNDS TCLLCDDCPGDINALPELKH YDLKQPTQPPPLPAAEAEKP ESSVSSATGDGNLSPSQPPL KNLLSLLKAYYALNAQPSAE ELSKIADSVNLPLDVVKKWF EKMQAGQISVQSSEPSSPEP GKVNIPAKNNDQPQSANANE PQDSTVNLQSPLKMTNSPVL PVGSTTNGSRSSTPSPSPLN L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 490-710 of human ZEB1 (NP_110378.3). |
Additional Info
| Tissue Specificity | Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. |
| Post Translational Modifications | Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization. |
| Function | Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region. |
| Protein Name | Zinc Finger E-Box-Binding Homeobox 1Nil-2-A Zinc Finger ProteinNegative Regulator Of Il2Transcription Factor 8Tcf-8 |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9926550 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger E-Box-Binding Homeobox 1 antibodyAnti-Nil-2-A Zinc Finger Protein antibodyAnti-Negative Regulator Of Il2 antibodyAnti-Transcription Factor 8 antibodyAnti-Tcf-8 antibodyAnti-ZEB1 antibodyAnti-AREB6 antibodyAnti-TCF8 antibody |
Information sourced from Uniprot.org