• Western blot analysis of extracts of HeLa cells, using WDR5 antibody (STJ115543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-WDR5 antibody (1-334) (STJ115543)

SKU:
STJ115543

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WDR5 (1-334) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDR5
Gene ID: 11091
Uniprot ID: WDR5_HUMAN
Immunogen Region: 1-334
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human WDR5 (NP_438172.1).
Immunogen Sequence: MATEEKKPETEAARAQPTPS SSATQSKPTPVKPNYALKFT LAGHTKAVSSVKFSPNGEWL ASSSADKLIKIWGAYDGKFE KTISGHKLGISDVAWSSDSN LLVSASDDKTLKIWDVSSGK CLKTLKGHSNYVFCCNFNPQ SNLIVSGSFDESVRIWDVKT GKCLKTLPAHSDPVSAVHFN RDGSLIVSSSYDGLCRIWDT ASGQCLKTLIDDDNPPVSFV KFSPNGKYILAATLDNTLK
Function Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Protein Name Wd Repeat-Containing Protein 5
Bmp2-Induced 3-Kb Gene Protein
Database Links Reactome: R-HSA-3214841
Reactome: R-HSA-3214847
Reactome: R-HSA-3214858
Reactome: R-HSA-5617472
Reactome: R-HSA-8936459
Reactome: R-HSA-8951664
Reactome: R-HSA-9733709
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Wd Repeat-Containing Protein 5 antibody
Anti-Bmp2-Induced 3-Kb Gene Protein antibody
Anti-WDR5 antibody
Anti-BIG3 antibody

Information sourced from Uniprot.org

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