Western blot analysis of extracts of mouse testis, using TSSK4 antibody (STJ110171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST.

Anti-TSSK4 antibody (79-328) (STJ110171)

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STJ110171
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSSK4
Gene ID: 283629
Uniprot ID: TSSK4_HUMAN
Immunogen Region: 79-328
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 79-328 of human TSSK4 (NP_777604.2).
Immunogen Sequence: VLRHKYLINFYRAIESTSRV YIILELAQGGDVLEWIQRYG ACSEPLAGKWFSQLTLGIAY LHSKSIVHRDLKLENLLLDK WENVKISDFGFAKMVPSNQP VGCSPSYRQVNCFSHLSQTY CGSFAYACPEILRGLPYNPF LSDTWSMGVILYTLVVAHLP FDDTNLKKLLRETQKEVTFP ANHTISQECKNLILQMLRQA TKRATILDIIKDSWVLKFQP EQPTHEIRLLEAMCQLHNT
Tissue Specificity Expressed only in the testis.
Post Translational Modifications Activated by autophosphorylation on Thr-197. ODF2 potentiates the autophosphorylation activity of TSSK4 at Thr-197. Ubiquitinated.HSP90 activity negatively regulates ubiquitination and degradation.
Function Serine/threonine kinase which is involved in male germ cell development and in mature sperm function. May be involved in the Cre/Creb signaling pathway. Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells. Phosphorylates CREM on 'Ser-116' in vitro. Phosphorylates ODF2 on 'Ser-95'.
Protein Name Testis-Specific Serine/Threonine-Protein Kinase 4
Tsk-4
Tssk-4
Testis-Specific Kinase 4
Serine/Threonine-Protein Kinase 22e
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cell Projection
Cilium
Flagellum
In Spermatozoa
Present In The Sperm Head And In The Flagellum
Alternative Antibody Names Anti-Testis-Specific Serine/Threonine-Protein Kinase 4 antibody
Anti-Tsk-4 antibody
Anti-Tssk-4 antibody
Anti-Testis-Specific Kinase 4 antibody
Anti-Serine/Threonine-Protein Kinase 22e antibody
Anti-TSSK4 antibody
Anti-C14orf20 antibody
Anti-STK22E antibody
Anti-TSSK5 antibody

Information sourced from Uniprot.org

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